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  Indian J Med Microbiol
 

Figure 4: Alkaloids from Hypecoum leptocarpum and protopine from Hypecoum leptocarpum inhibited mRNA expression of inflammatory cytokines in lipopolysaccharide-induced RAW264.7 cells. Cells were treated with lipopolysaccharide (1 μg/mL) and different concentrations (1.56, 6.25, or 25 mL) of alkaloids from Hypecoum leptocarpum or protopine for 24 h. mRNA levels of inducible nitric oxide synthase (a), interleukin-β (b), interleukin-6 (c), and tumor necrosis factor-a (d) were determined by reverse transcription-polymerase chain reaction. Data represent the mean ± standard deviation from three separate experiments. #P < 0.01, significant compared with vehicle-treated control; *P < 0.05, **P < 0.01 significant compared with lipopolysaccharide alone

Figure 4: Alkaloids from <i>Hypecoum leptocarpum</i> and protopine from <i>Hypecoum leptocarpum</i> inhibited mRNA expression of inflammatory cytokines in lipopolysaccharide-induced RAW264.7 cells. Cells were treated with lipopolysaccharide (1 μg/mL) and different concentrations (1.56, 6.25, or 25 mL) of alkaloids from <i>Hypecoum leptocarpum</i> or protopine for 24 h. mRNA levels of inducible nitric oxide synthase (a), interleukin-β (b), interleukin-6 (c), and tumor necrosis factor-a (d) were determined by reverse transcription-polymerase chain reaction. Data represent the mean ± standard deviation from three separate experiments. <sup>#</sup><i>P</i> < 0.01, significant compared with vehicle-treated control; *<i>P</i> < 0.05, **<i>P</i> < 0.01 significant compared with lipopolysaccharide alone