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  Indian J Med Microbiol
 

Figure 3: Alkaloids from Hypecoum leptocarpum and protopine from Hypecoum leptocarpum inhibited inflammatory cytokine release in lipopolysaccharide-induced RAW264.7 cells. (a) Viability of RAW264.7 cells, determined by 3-(4, 5-dimethylthylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay, following treatment with alkaloids from Hypecoum leptocarpum or protopine (12.5, 25, 50, 100, or 200 μg/mL) for 20 h. (b-e) Macrophages were treated with lipopolysaccharide (1 mL) and alkaloids from Hypecoum leptocarpum or protopine (1.56, 3.13, 6.25, 12.50, or 25 mL) for 24 h. The culture supernatants were collected and nitric oxide (b), interleukin-β (c), interleukin-6 (d), and tumor necrosis factor-a (e) were measured. Data represent the mean ± standard deviation from three separate experiments. #P < 0.01, significant compared with vehicle-treated control; *P < 0.05, **P < 0.01 significant compared with lipopolysaccharide alone

Figure 3: Alkaloids from <i>Hypecoum leptocarpum</i> and protopine from <i>Hypecoum leptocarpum</i> inhibited inflammatory cytokine release in lipopolysaccharide-induced RAW264.7 cells. (a) Viability of RAW264.7 cells, determined by 3-(4, 5-dimethylthylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay, following treatment with alkaloids from <i>Hypecoum leptocarpum</i> or protopine (12.5, 25, 50, 100, or 200 μg/mL) for 20 h. (b-e) Macrophages were treated with lipopolysaccharide (1 mL) and alkaloids from <i>Hypecoum leptocarpum</i> or protopine (1.56, 3.13, 6.25, 12.50, or 25 mL) for 24 h. The culture supernatants were collected and nitric oxide (b), interleukin-β (c), interleukin-6 (d), and tumor necrosis factor-a (e) were measured. Data represent the mean ± standard deviation from three separate experiments. <sup>#</sup><i>P</i> < 0.01, significant compared with vehicle-treated control; *<i>P</i> < 0.05, **<i>P</i> < 0.01 significant compared with lipopolysaccharide alone