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  Indian J Med Microbiol
 

Figure 3: Assessment of apoptosis in (a) LoVo and (b) HT-29 cell lines treated with the Rubus sanctus Schreb. root extract for 24 h. The cells cultured either in DMEM or in RPMI media were used as control. The apoptotic cells were detected by annexin V-PI dual staining. Upper-left quadrant (annexin V−, PI+) represented dead cells; upper-right quadrant (annexin V+, PI+) represented late apoptotic cells; lower-right quadrant (annexin V+, PI–) represented early apoptotic cells; lower-left quadrant (annexin V−, PI−) represented live cells. The percentage of total apoptotic cells (right quadrants) was calculated and shown in the bar graphs. The extent of apoptosis was significantly high both in cell lines. *P ≤ 0.05, **P ≤ 0.01

Figure 3: Assessment of apoptosis in (a) LoVo and (b) HT-29 cell lines treated with the <i>Rubus sanctus</i> Schreb. root extract for 24 h. The cells cultured either in DMEM or in RPMI media were used as control. The apoptotic cells were detected by annexin V-PI dual staining. Upper-left quadrant (annexin V−, PI+) represented dead cells; upper-right quadrant (annexin V+, PI+) represented late apoptotic cells; lower-right quadrant (annexin V+, PI–) represented early apoptotic cells; lower-left quadrant (annexin V−, PI−) represented live cells. The percentage of total apoptotic cells (right quadrants) was calculated and shown in the bar graphs. The extent of apoptosis was significantly high both in cell lines. *<i>P</i> ≤ 0.05, **<i>P</i> ≤ 0.01