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  Indian J Med Microbiol
 

Figure 6: The relative quantification of stress responsive and AD related genes in 5,7-dihydroxy-4-methoxyflavone (25 μM) exposed CL2006 worms. 5,7-dihydroxy-4-methoxyflavone treatment upregulates the expression level of genes sod-1, sod-2, sod-3, hsp-16.2, ctl-1 gst-4, unc-17, unc-50 and cha-1. β-actin (act-1) was used as endogenous control and the relative expression level was quantified using real time polymerase chain reaction using Ct (ΔΔCt) method. The data were statistically analyzed using ANOVA in ASSISTAT 7.7 beta statistical assistance software. Error bars represent means ± standard error of the mean. **P ≤ 0.001

Figure 6: The relative quantification of stress responsive and AD related genes in 5,7-dihydroxy-4-methoxyflavone (25 μM) exposed CL2006 worms. 5,7-dihydroxy-4-methoxyflavone treatment upregulates the expression level of genes sod-1, sod-2, sod-3, hsp-16.2, ctl-1 gst-4, unc-17, unc-50 and cha-1. β-actin (act-1) was used as endogenous control and the relative expression level was quantified using real time polymerase chain reaction using Ct (ΔΔCt) method. The data were statistically analyzed using ANOVA in ASSISTAT 7.7 beta statistical assistance software. Error bars represent means ± standard error of the mean. **<i>P</i> ≤ 0.001