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  Indian J Med Microbiol
 

Figure 4: (a) Effect of 5,7-dihydroxy-4-methoxyflavone treatments (5, 25 and 50 μM) on protein carbonyl contents in N2 worms. Age synchronized wild type worms were grown on OP-50 seeded nematode growth medium plate with or without of 5,7-dihydroxy-4-methoxyflavone. The 5,7-dihydroxy-4-methoxyflavone treated or nontreated day 5 N2 worms were used for the protein carbonylation assay. The 5,7-dihydroxy-4-methoxyflavone treatments were found to decrease the level of protein carbonyl contents in treated N2 worms as compared with control. Error Bars represent means ± standard error of the mean. **P ≤ 0.0001. (b) Effect of 5,7-dihydroxy-4-methoxyflavone treatments (5, 25 and 50 μM) on protein carbonyl contents in CL2006 worms. 5,7-dihydroxy-4-methoxyflavone treated (5, 25 and 50 μM) CL2006 worms were found to decrease the protein carbonyl content in comparison to control worms. The graph was plotted as relative change in protein carbonyl content in comparison to control at 100%. The data is statistically analyzed using ANOVA in ASSISTAT 7.7 beta statistical assistance software. Differences between the data were considered significant at P ≤ 0.05 error bars represent means ± standard error of the mean. **P ≤ 0.0001

Figure 4: (a) Effect of 5,7-dihydroxy-4-methoxyflavone treatments (5, 25 and 50 μM) on protein carbonyl contents in N2 worms. Age synchronized wild type worms were grown on OP-50 seeded nematode growth medium plate with or without of 5,7-dihydroxy-4-methoxyflavone. The 5,7-dihydroxy-4-methoxyflavone treated or nontreated day 5 N2 worms were used for the protein carbonylation assay. The 5,7-dihydroxy-4-methoxyflavone treatments were found to decrease the level of protein carbonyl contents in treated N2 worms as compared with control. Error Bars represent means ± standard error of the mean. **<i>P</i> ≤ 0.0001. (b) Effect of 5,7-dihydroxy-4-methoxyflavone treatments (5, 25 and 50 μM) on protein carbonyl contents in CL2006 worms. 5,7-dihydroxy-4-methoxyflavone treated (5, 25 and 50 μM) CL2006 worms were found to decrease the protein carbonyl content in comparison to control worms. The graph was plotted as relative change in protein carbonyl content in comparison to control at 100%. The data is statistically analyzed using ANOVA in ASSISTAT 7.7 beta statistical assistance software. Differences between the data were considered significant at <i>P</i> ≤ 0.05 error bars represent means ± standard error of the mean. **<i>P</i> ≤ 0.0001