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  Indian J Med Microbiol
 

Figure 3: Measurement of reactive oxygen species levels in 5,7-dihydroxy-4-methoxyflavone treated (5, 25 and 50 μM) CL2006 strain. The age synchronized day 4 treated and non-treated live worms were utilized to measure intracellular reactive oxygen species using 2,7-dichlorofluorescein diacetate dye at 37°C. The result exhibited low level of intracellular reactive oxygen species in 5,7-dihydroxy-4-methoxyflavone supplemented CL2006 worms as compared to vehicle control. The graph was plotted as relative change in reactive oxygen species compared to control at 100%. The data is statistically analyzed using ANOVA in ASSISTAT 7.7 beta statistical assistance software. Error bars represent means ± standard error of the mean. **P ≤ 0.0001

Figure 3: Measurement of reactive oxygen species levels in 5,7-dihydroxy-4-methoxyflavone treated (5, 25 and 50 μM) CL2006 strain. The age synchronized day 4 treated and non-treated live worms were utilized to measure intracellular reactive oxygen species using 2,7-dichlorofluorescein diacetate dye at 37°C. The result exhibited low level of intracellular reactive oxygen species in 5,7-dihydroxy-4-methoxyflavone supplemented CL2006 worms as compared to vehicle control. The graph was plotted as relative change in reactive oxygen species compared to control at 100%. The data is statistically analyzed using ANOVA in ASSISTAT 7.7 beta statistical assistance software. Error bars represent means ± standard error of the mean. **<i>P</i> ≤ 0.0001