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  Indian J Med Microbiol
 

Figure 2: (a) Effect of 5,7-dihydroxy-4-methoxyflavone on Aβ induced paralysis in CL4176 transgenic strain. Age synchronized CL4176 worms were fed with 5,7-dihydroxy-4-methoxyflavone (5, 25 and 50 μM) or vehicle control on nematode growth medium plate containing OP-50 as food source for 48 h prior to temperature up shift and scored for paralysis. To induce the transgene expression temperature was up shifted from 16°C to 25°C. 5,7-dihydroxy-4-methoxyflavone (5, 25 and 50 μM) treated CL4176 worms were found to be non-paralyzed in comparison to control worms. The data was processed using the Kaplan–Meir survival analysis in Medcalc 12.7.7.0 software. (b) PT50 values in hours (the time at which 50% of worms were paralyzed or dead after temperature upshift) for all 5,7-dihydroxy-4-methoxyflavone treatments (5, 25 and 50 μM) were calculated from paralysis curve as compared to untreated control. Differences between the data were considered significant at P ≤ 0.05. Error bars represent means ± standard error of the mean. **P ≤ 0.0001

Figure 2: (a) Effect of 5,7-dihydroxy-4-methoxyflavone on Aβ induced paralysis in CL4176 transgenic strain. Age synchronized CL4176 worms were fed with 5,7-dihydroxy-4-methoxyflavone (5, 25 and 50 μM) or vehicle control on nematode growth medium plate containing OP-50 as food source for 48 h prior to temperature up shift and scored for paralysis. To induce the transgene expression temperature was up shifted from 16°C to 25°C. 5,7-dihydroxy-4-methoxyflavone (5, 25 and 50 μM) treated CL4176 worms were found to be non-paralyzed in comparison to control worms. The data was processed using the Kaplan–Meir survival analysis in Medcalc 12.7.7.0 software. (b) PT<sub>50</sub> values in hours (the time at which 50% of worms were paralyzed or dead after temperature upshift) for all 5,7-dihydroxy-4-methoxyflavone treatments (5, 25 and 50 μM) were calculated from paralysis curve as compared to untreated control. Differences between the data were considered significant at <i>P</i> ≤ 0.05. Error bars represent means ± standard error of the mean. **<i>P</i> ≤ 0.0001