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  Indian J Med Microbiol
 

Figure 5: Fibrinolytic activity: (a) Colorimetric assay. (b) Dose-dependent effect. (c) Time-dependent effect. (d) Inhibition study. (a) Washed plasma clot was incubated with 0–100 μg of FSBE for 2.30 h and then the optical density was measured at 660 nm. (b) FSBE dose-dependent effect; washed plasma clot was incubated for 12 h and then separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis (7.5%), washed plasma clot alone (b1), plasma clot treated with 20 μg (b2), 40 μg (b3), 60 μg (b4), 80 μg (b5), and 100 μg (b6) of FSBE, respectively.(c) FSBE time-dependent effect; FSBE 40 μg was incubated with fibrin clot at 37°C, fibrin clot alone (c1), 0 h (c2), 6 h (c3), 12 h (c4), 18 h (c5), and 24 h (c6) of FSBE.(d) FSBE inhibition study: FSBE 40 μg was preincubated with protease inhibitors for 30 min at 37°C. Further reaction was initiated by adding fibrin clot and incubated for 12 h, fibrin clot alone (d1), FSBE 40 μg (d2), fibrin clot and FSBE 40 μg with 5 mM PMSF (d3), fibrin clot and FSBE 40 μg with 5 mM iodoacetic acid (d4), fibrin clot and FSBE 40 μg with 5 mM ethylenediaminetetraacetic acid (d5), fibrin clot and FSBE 40 μg with 5 mM 1,10-phenanthroline (d6). M represents the molecular-weight marker in kDa from top to bottom: Myosin-H-chain (200) phosphorylase b (97.2), BSA (66.4), ovalbumin (44.3), carbonic anhydrase (29), and lysozyme (14.3). BSA: Bovine serum albumin, EGTA: Ethylene di-amine tetra-acetic acid, IAA: Iodoacetic acid, PMSF: Phenylmethylsulfonyl fluoride, FSBE: Flaxseed buffer extract

Figure 5: Fibrinolytic activity: (a) Colorimetric assay. (b) Dose-dependent effect. (c) Time-dependent effect. (d) Inhibition study. (a) Washed plasma clot was incubated with 0–100 μg of FSBE for 2.30 h and then the optical density was measured at 660 nm. (b) FSBE dose-dependent effect; washed plasma clot was incubated for 12 h and then separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis (7.5%), washed plasma clot alone (b1), plasma clot treated with 20 μg (b2), 40 μg (b3), 60 μg (b4), 80 μg (b5), and 100 μg (b6) of FSBE, respectively.(c) FSBE time-dependent effect; FSBE 40 μg was incubated with fibrin clot at 37°C, fibrin clot alone (c1), 0 h (c2), 6 h (c3), 12 h (c4), 18 h (c5), and 24 h (c6) of FSBE.(d) FSBE inhibition study: FSBE 40 μg was preincubated with protease inhibitors for 30 min at 37°C. Further reaction was initiated by adding fibrin clot and incubated for 12 h, fibrin clot alone (d1), FSBE 40 μg (d2), fibrin clot and FSBE 40 μg with 5 mM PMSF (d3), fibrin clot and FSBE 40 μg with 5 mM iodoacetic acid (d4), fibrin clot and FSBE 40 μg with 5 mM ethylenediaminetetraacetic acid (d5), fibrin clot and FSBE 40 μg with 5 mM 1,10-phenanthroline (d6). M represents the molecular-weight marker in kDa from top to bottom: Myosin-H-chain (200) phosphorylase b (97.2), BSA (66.4), ovalbumin (44.3), carbonic anhydrase (29), and lysozyme (14.3). BSA: Bovine serum albumin, EGTA: Ethylene di-amine tetra-acetic acid, IAA: Iodoacetic acid, PMSF: Phenylmethylsulfonyl fluoride, FSBE: Flaxseed buffer extract