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  Indian J Med Microbiol
 

Figure 2: (a) Casein zymogram (b) Casein zymogram with inhibitor (c) Gelatin zymogram (d) Gelatin zymogram with inhibitor.(a) FSBE casein zymogram: FSBE 50 μg (a1) and 100 μg (a2) under nonreduced conditions.(b) FSBE Casein zymogram with inhibitor: FSBE 50 μg alone (b1), FSBE 50 μg was pretreated with 5 mM PMSF (b2), FSBE 50 μg was pretreated with 5 mM iodoacetic acid (b3), FSBE 50 μg was pretreated with 5 mM 1,10-phenanthroline (b4), FSBE 50 μg was pretreated with 5 mM ethylenediaminetetraacetic acid (b5) under nonreduced conditions.(c) FSBE gelatin zymogram: FSBE 50 μg (c1) and 100 μg (c2) under nonreduced conditions.(d) FSBE gelatin zymogram with inhibitor: FSBE 50 μg alone (d1), FSBE 50 μg was pretreated with 5 mM PMSF (d2), FSBE 50 μg was pretreated with 5 mM iodoacetic acid (d3), FSBE 50 μg was pretreated with 5 mM 1,10-phenanthroline (d4), FSBE 50 μg was pretreated with 5 mM ethylenediaminetetraacetic acid (d5) under nonreduced conditions. M represents the molecular-weight marker in kDa from top to bottom: Myosin-H-chain (200), BSA (66.4), ovalbumin (44.3), carbonic anhydrase (29), β-lactalbumin (18.4), and lysozyme (14.3). BSA: Bovine serum albumin, FSBE: Flaxseed buffer extract

Figure 2: (a) Casein zymogram (b) Casein zymogram with inhibitor (c) Gelatin zymogram (d) Gelatin zymogram with inhibitor.(a) FSBE casein zymogram: FSBE 50 μg (a1) and 100 μg (a2) under nonreduced conditions.(b) FSBE Casein zymogram with inhibitor: FSBE 50 μg alone (b1), FSBE 50 μg was pretreated with 5 mM PMSF (b2), FSBE 50 μg was pretreated with 5 mM iodoacetic acid (b3), FSBE 50 μg was pretreated with 5 mM 1,10-phenanthroline (b4), FSBE 50 μg was pretreated with 5 mM ethylenediaminetetraacetic acid (b5) under nonreduced conditions.(c) FSBE gelatin zymogram: FSBE 50 μg (c1) and 100 μg (c2) under nonreduced conditions.(d) FSBE gelatin zymogram with inhibitor: FSBE 50 μg alone (d1), FSBE 50 μg was pretreated with 5 mM PMSF (d2), FSBE 50 μg was pretreated with 5 mM iodoacetic acid (d3), FSBE 50 μg was pretreated with 5 mM 1,10-phenanthroline (d4), FSBE 50 μg was pretreated with 5 mM ethylenediaminetetraacetic acid (d5) under nonreduced conditions. M represents the molecular-weight marker in kDa from top to bottom: Myosin-H-chain (200), BSA (66.4), ovalbumin (44.3), carbonic anhydrase (29), β-lactalbumin (18.4), and lysozyme (14.3). BSA: Bovine serum albumin, FSBE: Flaxseed buffer extract