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  Indian J Med Microbiol
 

Figure 5: Effects of Spirulina maxima on total glutathione + glutathione disulfide amount (a) and activity of glutathione reductase (b) and glutathione peroxidase (c). Cells were pretreated with three different concentrations of Spirulina maxima (1, 10, or 100 μg/mL) and trolox for an hour followed by stimulation with 2.5 mM glutamate. The supernatant is collected and (a) glutathione + glutathione disulfide, (b) Glutathione reductase, and (c) Glutathione peroxidase were detected by microplate reader and each data are expressed by percentage absorbance. Data are presented as means ± standard deviation (n = 3, *P < 0.05, **P < 0.01, and ***P < 0.001 verses only glutamate-treated group)

Figure 5: Effects of <i>Spirulina maxima</i> on total glutathione + glutathione disulfide amount (a) and activity of glutathione reductase (b) and glutathione peroxidase (c). Cells were pretreated with three different concentrations of <i>Spirulina maxima</i> (1, 10, or 100 μg/mL) and trolox for an hour followed by stimulation with 2.5 mM glutamate. The supernatant is collected and (a) glutathione + glutathione disulfide, (b) Glutathione reductase, and (c) Glutathione peroxidase were detected by microplate reader and each data are expressed by percentage absorbance. Data are presented as means ± standard deviation (<i>n</i> = 3, *<i>P</i> < 0.05, **<i>P</i> < 0.01, and ***<i>P</i> < 0.001 verses only glutamate-treated group)