Figure 3: Effects of Spirulina. maxima on glutamate-induced intracellular reactive oxygen species accumulation (a) and Ca2+ influx (b). Cells were pretreated with three different concentrations of Spirulina maxima (1, 10, or 100 μg/mL) and trolox for an hour followed by stimulation with glutamate. Reactive oxygen species and Ca2+ were detected by fluorometer and expressed as relative fluorescence intensity. Data are presented as means ± standard deviation (n = 6, *P < 0.05, **P < 0.01, and ***P < 0.001 verses only glutamate-treated group)
