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  Indian J Med Microbiol
 

Figure 5: Induction of apoptosis by Td-F2 extracts in hepatoma cells. HepG2 cells were left untreated, treated with DMSO, various concentrations of Td-F2 extracts, or positive control 10 μM camptothecin (Cam) podophyllotoxin 20 μg/mL (Pod) for 24 h. After cells were lyzed, chromosomal DNA inside cells was purified and analyzed by agarose electrophoresis (A) or cells with apoptotic DNA (cytosolic histone-bound DNA fragments) were analyzed by the Cell Death Detection ELISAPLUS Kit (B). **P < 0.01 and ***P < 0.001 (t-test) as compared with untreated control

Figure 5: Induction of apoptosis by Td-F2 extracts in hepatoma cells. HepG2 cells were left untreated, treated with DMSO, various concentrations of Td-F2 extracts, or positive control 10 μM camptothecin (Cam) podophyllotoxin 20 μg/mL (Pod) for 24 h. After cells were lyzed, chromosomal DNA inside cells was purified and analyzed by agarose electrophoresis (A) or cells with apoptotic DNA (cytosolic histone-bound DNA fragments) were analyzed by the Cell Death Detection ELISAPLUS Kit (B). **<i>P</i> < 0.01 and ***<i>P</i> < 0.001 (<i>t</i>-test) as compared with untreated control