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  Indian J Med Microbiol
 

Figure 4: Induction of fragmented chromosomal DNA by Td-F2 extracts in hepatoma cells. HepG2 hepatoma cells were left untreated, treated with DMSO (solvent control), various concentrations of Td-F2 extracts as indicated, positive control podophyllotoxin 20 μg/mL (Pod) or 10 μM camptothecin (Cam) for 24 h. Cells were trypsinized, fixed, and stained with PI. Cell cycle distribution was analyzed by flow cytometry as described in “Material and Methods” section. Percent G0/G1 cells include cells with 2N chromosomal DNA and with DNA less than 2N (sub-G1)

Figure 4: Induction of fragmented chromosomal DNA by Td-F2 extracts in hepatoma cells. HepG2 hepatoma cells were left untreated, treated with DMSO (solvent control), various concentrations of Td-F2 extracts as indicated, positive control podophyllotoxin 20 μg/mL (Pod) or 10 μM camptothecin (Cam) for 24 h. Cells were trypsinized, fixed, and stained with PI. Cell cycle distribution was analyzed by flow cytometry as described in “Material and Methods” section. Percent G0/G1 cells include cells with 2N chromosomal DNA and with DNA less than 2N (sub-G1)