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  Indian J Med Microbiol
 

Figure 2: Melastoma malabathricum-mediated cell death at 24 h was not apoptotic. MCF-7 and A549 cells were fixed with 100% cold methanol at 24 h post-treatment (untreated, dimethyl sulfoxide or 500 μg/ml of Melastoma malabathricum). (a) Cell nuclei were stained with DAPI and observed under ×100 magnification using a fluorescence microscope. N, normal nuclei; NC, necrotic nuclei; AP, apoptotic nuclei. (b) Cells were terminal deoxynucleotidyl transferase dUTP nick-end labeling stained before measuring terminal deoxynucleotidyl transferase dUTP nick-end labeling -positive events by flow cytometry. Histogram plots show change in fluorescein intensity (terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells) over time post-Melastoma malabathricum treatment. Data are representative of three independent experiments

Figure 2: <i>Melastoma malabathricum</i>-mediated cell death at 24 h was not apoptotic. MCF-7 and A549 cells were fixed with 100% cold methanol at 24 h post-treatment (untreated, dimethyl sulfoxide or 500 μg/ml of <i>Melastoma malabathricum</i>). (a) Cell nuclei were stained with DAPI and observed under ×100 magnification using a fluorescence microscope. N, normal nuclei; NC, necrotic nuclei; AP, apoptotic nuclei. (b) Cells were terminal deoxynucleotidyl transferase dUTP nick-end labeling stained before measuring terminal deoxynucleotidyl transferase dUTP nick-end labeling -positive events by flow cytometry. Histogram plots show change in fluorescein intensity (terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells) over time post-<i>Melastoma malabathricum</i> treatment. Data are representative of three independent experiments