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  Indian J Med Microbiol
 

Figure 1: Melastoma malabathricum-induced cell death in MCF-7 and A549 cells occurs predominantly by secondary necrosis/late apoptosis. (a) MCF-7 and A549 cells were incubated with varying concentrations of Melastoma malabathricum over 24 h before measuring cell viability by 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Percentage cell viability was determined by comparing the effect of Melastoma malabathricum to the vehicle control (dimethyl sulfoxide). Data represents the mean ± standard error mean of four independent experiments. *P < 0.005, by Student's t-test. (b) MCF-7 and A549 cells were incubated with 500 μg/ml Melastoma malabathricum, dimethyl sulfoxide or no treatment for 24 h before staining with Annexin-V and PI and analysis by flow cytometry. Results are typical of two independent experiments. (c) Examples of (b) are shown, with graphs showing the mean ± range of two independent experiments

Figure 1: <i>Melastoma malabathricum</i>-induced cell death in MCF-7 and A549 cells occurs predominantly by secondary necrosis/late apoptosis. (a) MCF-7 and A549 cells were incubated with varying concentrations of <i>Melastoma malabathricum</i> over 24 h before measuring cell viability by 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Percentage cell viability was determined by comparing the effect of <i>Melastoma malabathricum</i> to the vehicle control (dimethyl sulfoxide). Data represents the mean ± standard error mean of four independent experiments. *<i>P</i> < 0.005, by Student's <i>t</i>-test. (b) MCF-7 and A549 cells were incubated with 500 μg/ml <i>Melastoma malabathricum</i>, dimethyl sulfoxide or no treatment for 24 h before staining with Annexin-V and PI and analysis by flow cytometry. Results are typical of two independent experiments. (c) Examples of (b) are shown, with graphs showing the mean ± range of two independent experiments