Close
  Indian J Med Microbiol
 

Figure 3: Chalepin induces changes expression in cell cycle-related proteins in a dose-dependent manner. A549 cells (2 × 106 mL-1) were treated with 36 μg/ml of chalepin at various time points, after which the whole-cell extracts were prepared and 30 μg of protein was resolved by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, electrotransferred onto nitrocellulose membranes, and probed for the cyclin E, cyclin D1, CDK2, CDK4, p21, p27, and phosphorylated retinoblastoma proteins. The experiments were conducted in triplicates

Figure 3: Chalepin induces changes expression in cell cycle-related proteins in a dose-dependent manner. A549 cells (2 × 10<sup>6</sup> mL<sup>-1</sup>) were treated with 36 μg/ml of chalepin at various time points, after which the whole-cell extracts were prepared and 30 μg of protein was resolved by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, electrotransferred onto nitrocellulose membranes, and probed for the cyclin E, cyclin D1, CDK2, CDK4, p21, p27, and phosphorylated retinoblastoma proteins. The experiments were conducted in triplicates