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  Indian J Med Microbiol
 

Figure 10: Chalepin promotes cleavage of BH3-interacting domain death agonist in A549 cell line in a time-dependent manner. A549 cells (2 × 106 mL-1) were treated with 36 μg/ml of chalepin at various time points, after which the whole-cell extracts were prepared and 30 μg of protein was resolved by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, electrotransferred onto nitrocellulose membranes, and probed for BH3-interacting domain death agonist protein. The experiments were conducted in triplicates

Figure 10: Chalepin promotes cleavage of BH3-interacting domain death agonist in A549 cell line in a time-dependent manner. A549 cells (2 × 10<sup>6</sup> mL<sup>-1</sup>) were treated with 36 μg/ml of chalepin at various time points, after which the whole-cell extracts were prepared and 30 μg of protein was resolved by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, electrotransferred onto nitrocellulose membranes, and probed for BH3-interacting domain death agonist protein. The experiments were conducted in triplicates