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  Indian J Med Microbiol
 

Figure 3: High-performance liquid chromatography chromatograms of the (a) crude n-butanol fraction and (b) collected compounds by high-speed countercurrent chromatography. High-performance liquid chromatography experimental condition: an Ascentis® Express C18 column (150 mm × 4.6 mm, I.D., 2.7 μm, Supelco Analytical, USA), with the following gradient system was used: water with 0.5% formic acid (v/v) (a) and acetonitrile (b); gradient program: 10% B to 90% B, 0–25 min; 90% B to 10% B. Flow rate was set to be at 0.3 mL/min. All samples were injected with the volume of 10 μL

Figure 3: High-performance liquid chromatography chromatograms of the (a) crude n-butanol fraction and (b) collected compounds by high-speed countercurrent chromatography. High-performance liquid chromatography experimental condition: an Ascentis® Express C18 column (150 mm × 4.6 mm, I.D., 2.7 μm, Supelco Analytical, USA), with the following gradient system was used: water with 0.5% formic acid (v/v) (a) and acetonitrile (b); gradient program: 10% B to 90% B, 0–25 min; 90% B to 10% B. Flow rate was set to be at 0.3 mL/min. All samples were injected with the volume of 10 μL