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  Indian J Med Microbiol
 

Figure 2: Effects of berberine on phosphorylation of myosin by myosin light-chain kinase (x̄ ± s, n = 6). The assays are performed in the assay condition described in Materials and Methods. (a) Glycerol electrophoresis results of 20 kDa myosin light chain of myosin phosphorylated by myosin light-chain kinase, LC20, unphosphorylated 20 kDa myosin light chain; p-LC20, monophosphorylated 20 kDa regulating myosin light chain; LC17, 17 KDa myosin essential light chains. Lane 0 is unphosphorylation control, lane 1 is phosphorylation of myosin without berberine administrated, lane 2–6 represent phosphorylation of myosin after 6.25, 12.5, 25, 50, 100 μmol/L berberine administrated. (b) The extent of myosin phosphorylation, which is analyzed using Scion Image Software. Monophosphorylation is chosen as the control and calculated as 100%, and other data are the relative values compared to the control. *P < 0.05, **P < 0.01 versus phosphorylation without berberine

Figure 2: Effects of berberine on phosphorylation of myosin by myosin light-chain kinase (x̄ ± s, <i>n</i> = 6). The assays are performed in the assay condition described in Materials and Methods. (a) Glycerol electrophoresis results of 20 kDa myosin light chain of myosin phosphorylated by myosin light-chain kinase, LC<sub>20</sub>, unphosphorylated 20 kDa myosin light chain; p-LC<sub>20</sub>, monophosphorylated 20 kDa regulating myosin light chain; LC<sub>17</sub>, 17 KDa myosin essential light chains. Lane 0 is unphosphorylation control, lane 1 is phosphorylation of myosin without berberine administrated, lane 2–6 represent phosphorylation of myosin after 6.25, 12.5, 25, 50, 100 μmol/L berberine administrated. (b) The extent of myosin phosphorylation, which is analyzed using Scion Image Software. Monophosphorylation is chosen as the control and calculated as 100%, and other data are the relative values compared to the control. *<i>P</i> < 0.05, **<i>P</i> < 0.01 versus phosphorylation without berberine