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  Indian J Med Microbiol
 

Figure 5: Involvement of JAK2/STAT3 signaling. (a) RAW 264.7 cells were treated with lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50 and 100 μg/mL) and harvest at 4 h posttreatment. Cells treated with dimethyl sulfoxide were set as control. p-JAK2, JAK2, p-STAT3, and STAT3 were detected by Western blotting. Representative Western blots (left panel) and quantitative results (right panel) were shown. All values are means ± standard deviation (n = 3). &&& P < 0.001 versus control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus lipopolysaccharide-treated cells; #P < 0.05 and ##P < 0.01 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; $P < 0.05 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells. (b-d) RAW 264.7 cells were infected with STAT3 expression lentivirus or control vector lentivirus (Vector). After 24 h, cells were treated with lipopolysaccharide (1 μg/mL), and dimethyl sulfoxide or 100 μg/mL prim-O-glucosylcimifugin. The amount of nitrite (b) and cytokines (c) in the medium was monitored at 24 h after exposure. Protein expression of inducible nitric oxide synthase and cyclooxygenase 2 (d) in RAW 264.7 cells was detected by Western blotting. **P < 0.01 and ***P < 0.001 versus Vector + lipopolysaccharide; #P < 0.05 and ###P < 0.001 versus STAT3 + lipopolysaccharide; $P < 0.05, $$P < 0.01, and $$$P < 0.001 versus POS + lipopolysaccharide

Figure 5: Involvement of JAK2/STAT3 signaling. (a) RAW 264.7 cells were treated with lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50 and 100 μg/mL) and harvest at 4 h posttreatment. Cells treated with dimethyl sulfoxide were set as control. p-JAK2, JAK2, p-STAT3, and STAT3 were detected by Western blotting. Representative Western blots (left panel) and quantitative results (right panel) were shown. All values are means ± standard deviation (<i>n</i> = 3). <sup>&&&</sup> <i>P</i> < 0.001 versus control; *<i>P</i> < 0.05, **<i>P</i> < 0.01, and ***<i>P</i> < 0.001 versus lipopolysaccharide-treated cells; <sup>#</sup><i>P</i> < 0.05 and <sup>##</sup><i>P</i> < 0.01 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; <sup>$</sup><i>P</i> < 0.05 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells. (b-d) RAW 264.7 cells were infected with STAT3 expression lentivirus or control vector lentivirus (Vector). After 24 h, cells were treated with lipopolysaccharide (1 μg/mL), and dimethyl sulfoxide or 100 μg/mL prim-O-glucosylcimifugin. The amount of nitrite (b) and cytokines (c) in the medium was monitored at 24 h after exposure. Protein expression of inducible nitric oxide synthase and cyclooxygenase 2 (d) in RAW 264.7 cells was detected by Western blotting. **<i>P</i> < 0.01 and ***<i>P</i> < 0.001 versus Vector + lipopolysaccharide; <sup>#</sup><i>P</i> < 0.05 and <sup>###</sup><i>P</i> < 0.001 versus STAT3 + lipopolysaccharide; <sup>$</sup><i>P</i> < 0.05, <sup>$$</sup><i>P</i> < 0.01, and <sup>$$$</sup><i>P</i> < 0.001 versus POS + lipopolysaccharide