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  Indian J Med Microbiol
 

Figure 4: Effects of prim-O-glucosylcimifugin on the inducible nitric oxide synthase and cyclooxygenase 2 expressions of lipopolysaccharide-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50, and 100 μg/mL) and harvest at 24 h posttreatment. Cells treated with dimethyl sulfoxide only were set as control. (a) mRNA expression of inducible nitric oxide synthase and cyclooxygenase 2 was detected by real-time polymerase chain reaction with GAPDH as internal control; All values are means ± standard deviation (n = 3). (b) Protein expression of inducible nitric oxide synthase and cyclooxygenase 2 was detected by Western blotting. Representative Western blots (upper panel) and quantitative results (lower panel) were shown. All values are means ± standard deviation (n = 3). &&& P < 0.001 versus control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus lipopolysaccharide-treated cells; #P < 0.05, ##P < 0.01, and ###P < 0.001 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; $P < 0.05, $$P < 0.01, and $$$P < 0.001 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells

Figure 4: Effects of prim-O-glucosylcimifugin on the inducible nitric oxide synthase and cyclooxygenase 2 expressions of lipopolysaccharide-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50, and 100 μg/mL) and harvest at 24 h posttreatment. Cells treated with dimethyl sulfoxide only were set as control. (a) mRNA expression of inducible nitric oxide synthase and cyclooxygenase 2 was detected by real-time polymerase chain reaction with GAPDH as internal control; All values are means ± standard deviation (<i>n</i> = 3). (b) Protein expression of inducible nitric oxide synthase and cyclooxygenase 2 was detected by Western blotting. Representative Western blots (upper panel) and quantitative results (lower panel) were shown. All values are means ± standard deviation (<i>n</i> = 3). <sup>&&&</sup> <i>P</i> < 0.001 versus control; *<i>P</i> < 0.05, **<i>P</i> < 0.01, and ***<i>P</i> < 0.001 versus lipopolysaccharide-treated cells; <sup>#</sup><i>P</i> < 0.05, <sup>##</sup><i>P</i> < 0.01, and <sup>###</sup><i>P</i> < 0.001 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; <sup>$</sup><i>P</i> < 0.05, <sup>$$</sup><i>P</i> < 0.01, and <sup>$$$</sup><i>P</i> < 0.001 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells