Close
  Indian J Med Microbiol
 

Figure 3: Effects of prim-O-glucosylcimifugin on lipopolyssacharide-induced NO and cytokine production. Raw 264.7 cells were incubated in a medium containing lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50, and 100 μg/mL). Cells treated with dimethyl sulfoxide were set as control. The amount of nitrite (a), tumor necrosis factor-a (b), interleukin-6 (c), and interleukin-1β (d) in the medium was monitored at 24 h after exposure as described in Materials and Methods. All values are means ± standard deviation (n = 3). &&& P < 0.001 versus control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus lipopolysaccharide-treated cells; #P < 0.05, ##P < 0.01, and ###P < 0.001 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; $$P < 0.01 and $$$P < 0.001 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells

Figure 3: Effects of prim-O-glucosylcimifugin on lipopolyssacharide-induced NO and cytokine production. Raw 264.7 cells were incubated in a medium containing lipopolysaccharide (1 μg/mL) and various concentrations of prim-O-glucosylcimifugin (15, 50, and 100 μg/mL). Cells treated with dimethyl sulfoxide were set as control. The amount of nitrite (a), tumor necrosis factor-a (b), interleukin-6 (c), and interleukin-1β (d) in the medium was monitored at 24 h after exposure as described in Materials and Methods. All values are means ± standard deviation (<i>n</i> = 3). <sup>&&&</sup> <i>P</i> < 0.001 versus control; *<i>P</i> < 0.05, **<i>P</i> < 0.01, and ***<i>P</i> < 0.001 versus lipopolysaccharide-treated cells; <sup>#</sup><i>P</i> < 0.05, <sup>##</sup><i>P</i> < 0.01, and <sup>###</sup><i>P</i> < 0.001 versus lipopolysaccharide and 15 μg/mL prim-O-glucosylcimifugin-treated cells; <sup>$$</sup><i>P</i> < 0.01 and <sup>$$$</sup><i>P</i> < 0.001 versus lipopolysaccharide and 50 μg/mL prim-O-glucosylcimifugin-treated cells