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  Indian J Med Microbiol
 

Figure 1: Representative HPLC chromatogram of H. dulcis fruit extracts. An Xbridge™ Shield RP18 column (4.6 mm I.D. × 150 mm, 3.5 μm) (Waters, Milford, USA) was used for the chromatographic separation. Column oven temperature maintained at 30°C. The mobile phase was composed of 0.1% acetic acid (solvent A) and acetonitrile (solvent B). The flow rate was 1.0 mL/min. The composition of solvent B was maintained at 20% for 3 min, linearly increased to 38% for 24 min, further increased to 90% for 1 min and maintained at 90% for 5 min, which was followed by equilibration to the initial composition for 6 min. The injection volume was 10 μL and the UV detection wavelength was 290 nm.

Figure 1: Representative HPLC chromatogram of <i>H. dulcis</i> fruit extracts. An Xbridge™ Shield RP18 column (4.6 mm I.D. × 150 mm, 3.5 μm) (Waters, Milford, USA) was used for the chromatographic separation. Column oven temperature maintained at 30°C. The mobile phase was composed of 0.1% acetic acid (solvent A) and acetonitrile (solvent B). The flow rate was 1.0 mL/min. The composition of solvent B was maintained at 20% for 3 min, linearly increased to 38% for 24 min, further increased to 90% for 1 min and maintained at 90% for 5 min, which was followed by equilibration to the initial composition for 6 min. The injection volume was 10 μL and the UV detection wavelength was 290 nm.