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  Indian J Med Microbiol
 

Figure 1: Effect of Etlingera pavieana fractions in lipopolysaccharide-stimulated RAW 264.7 macrophages. (a) Cells were co-incubated with 50 μμg/mL of each fraction of Etlingera pavieana and LPS (1 μg/mL) for 24 h. Culture supernatants were collected subsequently and analyzed for nitrite production. Percentage inhibition of nitric oxide production from each treatment is given in relation to nitrite concentration of lipopolysaccharide-stimulated RAW264.7 macrophage cells. ***P < 0.001 versus lipopolysaccharide alone. (b) Viability of cells harvested 24 h after treatment with each fraction of Etlingera pavieana was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Each column shows the mean ± standard deviation of three independent experiments with triplicate samples. CON: Unstimulated control cells, LPS: Lipopolysaccharide-stimulated cell, EPH: hexane fraction, EPE: Ethyl acetate fraction, EPW: Water fraction. (a: 730 mm × 840 mm; b: 741 mm × 421 mm)

Figure 1: Effect of <i>Etlingera pavieana</i> fractions in lipopolysaccharide-stimulated RAW 264.7 macrophages. (a) Cells were co-incubated with 50 μμg/mL of each fraction of Etlingera pavieana and LPS (1 μg/mL) for 24 h. Culture supernatants were collected subsequently and analyzed for nitrite production. Percentage inhibition of nitric oxide production from each treatment is given in relation to nitrite concentration of lipopolysaccharide-stimulated RAW264.7 macrophage cells. ***<i>P</i> < 0.001 versus lipopolysaccharide alone. (b) Viability of cells harvested 24 h after treatment with each fraction of Etlingera pavieana was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Each column shows the mean ± standard deviation of three independent experiments with triplicate samples. CON: Unstimulated control cells, LPS: Lipopolysaccharide-stimulated cell, EPH: hexane fraction, EPE: Ethyl acetate fraction, EPW: Water fraction. (a: 730 mm × 840 mm; b: 741 mm × 421 mm)