Figure 3: Representative phase contrast images of muscle precursor cells (a) unexposed control cells, (b) exposed to LPS (100 µg/ml), (c) exposed to LPS (100 µg/mL), pre-treated with CAP (50 µM). (White arrow indicates the undamaged cells; white arrow head indicates damaged rounded structures). Representative confocal images of myoblasts (d) unexposed control (e) exposed to LPS (f) exposed to LPS (100 μg/mL) and pretreated with CAP (50 µM). Dead cells are labeled by EB and have red nuclei. Live cells are labeled by AO and have green nuclei. (Dotted arrow indicates the dead cells while arrow indicates the living cells). Microscopic images [Figure 3]g, h, i magnification: ×40; [Figure 3]j, k, l, magnification: ×100) show the intracellular ROS in myoblastcells. AO, ; CAP, Capsaicin; EB, ; LPS, lipopolysaccharide; ROS, reactive oxygen species.