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  Indian J Med Microbiol
 

Figure 3: Effects of herbal extracts on DHT-activated androgenic activity of herbal extracts in 22Rv1/MMLV cells. (A) Trichosanthes kirilowii (TK) and Asarum sieboldii (AS). (B) Sanguisorba officinalis (SO) and Xanthium strumarium (XS). Cells were transiently transfected with apRL-TK vector and incubated for 24 h with various concentrations (10, 100 or 500 μg/mL) of each herbal extract in the presence of DHT (1 nM). The cell lysates were prepared for luciferase activity assay as described in the methodology above. Transfection efficiency for luciferase assay was normalized to the Renilla luciferase activity. The bar graphs represent the means ± SD from three independent experiments

Figure 3: Effects of herbal extracts on DHT-activated androgenic activity of herbal extracts in 22Rv1/MMLV cells. (A) <i>Trichosanthes kirilowii</i> (TK) and <i>Asarum sieboldii</i> (AS). (B) <i>Sanguisorba officinalis</i> (SO) and <i>Xanthium strumarium</i> (XS). Cells were transiently transfected with apRL-TK vector and incubated for 24 h with various concentrations (10, 100 or 500 μg/mL) of each herbal extract in the presence of DHT (1 nM). The cell lysates were prepared for luciferase activity assay as described in the methodology above. Transfection efficiency for luciferase assay was normalized to the Renilla luciferase activity. The bar graphs represent the means ± SD from three independent experiments