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  Indian J Med Microbiol
 

Figure 2: Androgenic effects of herbal extracts in 22Rv1/MMLV cells. (A) Trichosanthes kirilowii (TK) and Asarum sieboldii (AS). (B) Sanguisorba officinalis (SO) and Xanthium strumarium (XS). Cells were transiently transfected with a pRL-TK vector and incubated for 24 h with various concentrations (10, 100, 250 or 500 μg/mL) of each herbal extract. The cell lysates were prepared for luciferase activity assay as described in the methodology above. Transfection efficiency for luciferase assay was normalized to the Renilla luciferase activity. DHT (10 nM) was used as a positive control. The bar graphs represent the means ± SD from three independent experiments

Figure 2: Androgenic effects of herbal extracts in 22Rv1/MMLV cells. (A) <i>Trichosanthes kirilowii</i> (TK) and <i>Asarum sieboldii</i> (AS). (B) <i>Sanguisorba officinalis</i> (SO) and <i>Xanthium strumarium</i> (XS). Cells were transiently transfected with a pRL-TK vector and incubated for 24 h with various concentrations (10, 100, 250 or 500 μg/mL) of each herbal extract. The cell lysates were prepared for luciferase activity assay as described in the methodology above. Transfection efficiency for luciferase assay was normalized to the Renilla luciferase activity. DHT (10 nM) was used as a positive control. The bar graphs represent the means ± SD from three independent experiments