Figure 5: Effects of BPP on adipokine gene expression in 3T3-L1 by quantitative real-time RT-PCR analysis. Confluent 3T3-L1 pre-adipocytes were differentiated into adipocytes in a medium with BPP (0, 10, 25, and 50 μM) for 2 or 8 days. The mRNA expression levels of PPAR., C/EBPα SREBP1, SCD-1, FAS, aP2, LPL, and adiponectin on day 8 (A), and C/EBPβ on day 2 (B) was estimated by quantitative real-time RT-PCR analysis. Results represent the mean ± SD of three independent experiments, each performed in triplicate wells. * p < 0.05, ** p < 0.01, ***p < 0.001 versus differentiated cells.