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  Indian J Med Microbiol
 

Figure 2: (a) Two percent agarose gel is showing results of multiplex polymerase chain reaction. Lane 2, Lane 3, and Lane 4 specific to Terminalia arjuna, Terminalia bellirica, and Terminalia chebula, respectively, in control reactions having all the three primers but DNA of only one species. Lanes 5, 6, and 7 are showing polymerase chain reaction results of control reactions having all three primers with DNA of two species. In Lane 8, all the three amplicons are found detectable upon mixing of DNA of all the three species. (b) Sensitivity testing of multiplex polymerase chain reaction assay. Lanes 2 to 8 are showing amplification from of each species having DNA concentrations 2, 5, 10, 25, 50, 75, and 100 ng, respectively. Lane 1: No template control; M: GeneRuler 50bp DNA Ladder (Thermo Scientific, Massachuetts, United States)

Figure 2: (a) Two percent agarose gel is showing results of multiplex polymerase chain reaction. Lane 2, Lane 3, and Lane 4 specific to <i>Terminalia arjuna</i>, <i>Terminalia bellirica</i>, and <i>Terminalia chebula</i>, respectively, in control reactions having all the three primers but DNA of only one species. Lanes 5, 6, and 7 are showing polymerase chain reaction results of control reactions having all three primers with DNA of two species. In Lane 8, all the three amplicons are found detectable upon mixing of DNA of all the three species. (b) Sensitivity testing of multiplex polymerase chain reaction assay. Lanes 2 to 8 are showing amplification from of each species having DNA concentrations 2, 5, 10, 25, 50, 75, and 100 ng, respectively. Lane 1: No template control; M: GeneRuler 50bp DNA Ladder (Thermo Scientific, Massachuetts, United States)