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  Indian J Med Microbiol
 

Figure 2: Lane A-E (a) Coli topoisomerase I inhibition by extracts of Chonemorpha fragrans; (b) human topoisomerase I inhibition; (c) human topoisomerase II inhibition, (reproduction size 11.9 cm × 15.4 cm) Lane A: Supercolied pUC 19 DNA, Lane B: DNA + topoisomerase enzyme, Lane C: 1 μg camptothecin, Lane D: 0.1% dimethyl sulfoxide, Lane E-X: DNA + topoisomerase + 2 μg/test compound, E-H: Root MeOH, EtOAc, CH3Cl, C6H12 extracts resp., Lane I-L: Bark MeOH, EtOAc, CH3Cl, C6H12 extracts, respectively, Lane M-P: Lvs MeOH, EtOAc, CH3Cl, C6H12 extracts, respectively, Lane Q-T: In vitro MeOH, EtOAc, CH3Cl, C6H12 extracts, respectively, Lane U-X: Callus MeOH, EtOAc, CH3Cl, C6H12 extracts, respectively. (R) Relaxed and (S) supercoiled plasmids

Figure 2: Lane A-E (a) Coli topoisomerase I inhibition by extracts of <i>Chonemorpha fragrans</i>; (b) human topoisomerase I inhibition; (c) human topoisomerase II inhibition, (reproduction size 11.9 cm × 15.4 cm) Lane A: Supercolied pUC 19 DNA, Lane B: DNA + topoisomerase enzyme, Lane C: 1 μg camptothecin, Lane D: 0.1% dimethyl sulfoxide, Lane E-X: DNA + topoisomerase + 2 μg/test compound, E-H: Root MeOH, EtOAc, CH<sub>3</sub>C<sub>l</sub>, C<sub>6</sub>H<sub>12</sub> extracts resp., Lane I-L: Bark MeOH, EtOAc, CH<sub>3</sub>C<sub>l</sub>, C<sub>6</sub>H<sub>12</sub> extracts, respectively, Lane M-P: Lvs MeOH, EtOAc, CH<sub>3</sub>C<sub>l</sub>, C<sub>6</sub>H<sub>12</sub> extracts, respectively, Lane Q-T: <i>In vitro</i> MeOH, EtOAc, CH<sub>3</sub>C<sub>l</sub>, C<sub>6</sub>H<sub>12</sub> extracts, respectively, Lane U-X: Callus MeOH, EtOAc, CH<sub>3</sub>C<sub>l</sub>, C<sub>6</sub>H<sub>12</sub> extracts, respectively. (R) Relaxed and (S) supercoiled plasmids