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  Indian J Med Microbiol
 

Figure 4: Diosgenin induces cell survival. H9c2 cells were cultured in (1 × 104 in a 96-well plate). H9c2 cells were treated with 30 μM of diosgenin, followed by hypoxia and reoxygenation. The release of lactate dehydrogenase as a marker of cell death was performed with lactate dehydrogenase cytotoxicity assay kit (Cayman Chemical Company, Ann Arbor, MI, USA). Results are expressed as mean ± standard error of the mean *P = 0.05 versus Normal, ethanol + hypoxia-reoxygenation and Dios + hypoxia-reoxygenation; #P = 0.05 versus hypoxia-reoxygenation

Figure 4: Diosgenin induces cell survival. H9c2 cells were cultured in (1 &#215; 10<sup>4</sup> in a 96-well plate). H9c2 cells were treated with 30 μM of diosgenin, followed by hypoxia and reoxygenation. The release of lactate dehydrogenase as a marker of cell death was performed with lactate dehydrogenase cytotoxicity assay kit (Cayman Chemical Company, Ann Arbor, MI, USA). Results are expressed as mean &#177; standard error of the mean *<i>P</i> = 0.05 versus Normal, ethanol + hypoxia-reoxygenation and Dios + hypoxia-reoxygenation; #<i>P</i> = 0.05 versus hypoxia-reoxygenation