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  Indian J Med Microbiol
 

Figure 5: Inhibition of Hedgehog signaling pathway by Phenolic alkaloids from Menispermum dauricum (PAMD). (A) and (C) immunohistochemistry showed Shh and Gli-1 levels in the BxPC-3 tumor tissues. (a) Control group; (b) 5-Fu (20 mg/Kg) treated group; (c) PAMD (10 mg/Kg) treated group; (d) PAMD (20 mg/Kg) treated group. (B) and (D) integrated optical density of immunohistochemistry analyzed by ImagePro Plus. (E) and (F) Western blot showed Sonic Hedgehog (Shh) and Gli-1 protein levels. (G) and (H) qRT PCR of Shh and Gli-1. Results are presented as means ± standard deviation of three independent experiments. *P < 0.05: Significant difference; **P < 0.01: Very significant difference

Figure 5: Inhibition of Hedgehog signaling pathway by Phenolic alkaloids from <i>Menispermum dauricum</i> (PAMD). (A) and (C) immunohistochemistry showed Shh and Gli-1 levels in the BxPC-3 tumor tissues. (a) Control group; (b) 5-Fu (20 mg/Kg) treated group; (c) PAMD (10 mg/Kg) treated group; (d) PAMD (20 mg/Kg) treated group. (B) and (D) integrated optical density of immunohistochemistry analyzed by ImagePro Plus. (E) and (F) Western blot showed Sonic Hedgehog (Shh) and Gli-1 protein levels. (G) and (H) qRT PCR of Shh and Gli-1. Results are presented as means ± standard deviation of three independent experiments. *<i>P</i> < 0.05: Significant difference; **<i>P</i> < 0.01: Very significant difference