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  Indian J Med Microbiol
 

Figure 2: Phenolic alkaloids from Menispermum dauricum (PAMD) induces BxPC-3 pancreatic cancer cells G0/G1 phase arrest and induces apoptosis. (a) and (b) BxPC-3 cells were treated with PAMD, 5-fluoro-2, 4 (1 H, 3 H) pyrimidinedione (5-Fu) or saline for 24 h. The cells were then fixed with 70% ethanol at −20C overnight, incubated with RNase A at 37C for 30 min, stained with propidium iodide for 10 min, and analyzed with flow cytometry. (c) and (d) BxPC-3 cells were treated with PAMD, 5-Fu or saline for 24 h, and apoptotic cell death was detected by staining cells with an Annexin-V: FITC Apoptosis Detection Kit for analysis with flow cytometry

Figure 2: Phenolic alkaloids from <i>Menispermum dauricum</i> (PAMD) induces BxPC-3 pancreatic cancer cells G0/G1 phase arrest and induces apoptosis. (a) and (b) BxPC-3 cells were treated with PAMD, 5-fluoro-2, 4 (1 H, 3 H) pyrimidinedione (5-Fu) or saline for 24 h. The cells were then fixed with 70% ethanol at −20C overnight, incubated with RNase A at 37C for 30 min, stained with propidium iodide for 10 min, and analyzed with flow cytometry. (c) and (d) BxPC-3 cells were treated with PAMD, 5-Fu or saline for 24 h, and apoptotic cell death was detected by staining cells with an Annexin-V: FITC Apoptosis Detection Kit for analysis with flow cytometry