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  Indian J Med Microbiol
 

Figure 6: Determination of intracellular reactive oxygen species by 2',7'-dichlorofluorescein diacetate dye. (a) Control; (b) Flavonoid-enriched fraction control; (c) Ultraviolet-B control; (d) Positive control; (e) Ultraviolet-B-irradiated fibroblasts + 10 μg/ml fraction; (f) Ultraviolet –B-irradiated fibroblasts + 20 μg/ml fraction; (g) Ultraviolet-B-irradiated fibroblasts + 40 μg/ml fraction; and (h) Comparative graph showing % reactive oxygen species positive cells in treatment groups. Results were statistically analyzed using GraphPad Prism software by one-way analysis of variance followed by Dunnett's posttest and unpaired t-test to compare in-between the groups, *P < 0.05, ***P < 0.001

Figure 6: Determination of intracellular reactive oxygen species by 2',7'-dichlorofluorescein diacetate dye. (a) Control; (b) Flavonoid-enriched fraction control; (c) Ultraviolet-B control; (d) Positive control; (e) Ultraviolet-B-irradiated fibroblasts + 10 μg/ml fraction; (f) Ultraviolet –B-irradiated fibroblasts + 20 μg/ml fraction; (g) Ultraviolet-B-irradiated fibroblasts + 40 μg/ml fraction; and (h) Comparative graph showing % reactive oxygen species positive cells in treatment groups. Results were statistically analyzed using GraphPad Prism software by one-way analysis of variance followed by Dunnett's posttest and unpaired t-test to compare in-between the groups, <sup>*</sup><i>P</i> < 0.05, <sup>***</sup><i>P</i> < 0.001