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  Indian J Med Microbiol
 

Figure 2: Experimental schedule for restraint stress measuring wheel-running activity and treatment administration (a), and the effects of young green barley leaves on voluntary behavior measured by wheel-running activity in female mice subjected to restraint stress (b). Wheel-running activity was evaluated by running distance measured as the total number of wheel rotations in 16 h (1730-0930 h). Results are expressed as mean ± standard error of the mean for n = 7-9 mice. *P < 0.05 and ***P < 0.001 versus nonstressed (Nil) group on day 3 (Tukey-Kramer test). Vehicle: Distilled water; YG400 and YG1000: Young green barley leaf extract at doses of 400 mg/kg and 1,000 mg/kg, respectively; Clo: Clotiazepam at a dose of 10 mg/kg. The mean wheel-running counts in each experimental group on day-1 were: Nil: 17474.6 ± 1765.0; Vehicle: 17325.0 ± 1200.2; YG400: 16233.5 ± 2204.1; YG1000: 18153.0 ± 1846.3; and Clo: 17154.1 ± 1565.3

Figure 2: Experimental schedule for restraint stress measuring wheel-running activity and treatment administration (a), and the effects of young green barley leaves on voluntary behavior measured by wheel-running activity in female mice subjected to restraint stress (b). Wheel-running activity was evaluated by running distance measured as the total number of wheel rotations in 16 h (1730-0930 h). Results are expressed as mean ± standard error of the mean for <i>n</i> = 7-9 mice. *<i>P</i> < 0.05 and ***<i>P</i> < 0.001 versus nonstressed (Nil) group on day 3 (Tukey-Kramer test). Vehicle: Distilled water; YG400 and YG1000: Young green barley leaf extract at doses of 400 mg/kg and 1,000 mg/kg, respectively; Clo: Clotiazepam at a dose of 10 mg/kg. The mean wheel-running counts in each experimental group on day-1 were: Nil: 17474.6 ± 1765.0; Vehicle: 17325.0 ± 1200.2; YG400: 16233.5 ± 2204.1; YG1000: 18153.0 ± 1846.3; and Clo: 17154.1 ± 1565.3