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  Indian J Med Microbiol
 

Figure 5: MDR-1 (ABC-B1) inhibitory effects of enriched fraction of Elephantopus scaber (ES-DCM) in human epithelial cancer cells. HeLa (a) and Caco-2 (b) cells were incubated with rhodamine 123 (Rho-123) in Dulbecco's modified eagle medium with 2% fetal bovine serum for 30 min at 37°C (accumulation phase), after which excess dye was washed and cells were re-incubated with vehicle control (dimethyl sulfoxide), verapamil, and ES-DCM for 60 min at 37°C (efflux phase). The mean cellular Rho-123 fluorescence in the efflux phase was analyzed by flow cytometry. Error bars represent ± standard error of the mean; n = 3, ***P < 0.001

Figure 5: MDR-1 (ABC-B1) inhibitory effects of enriched fraction of <i>Elephantopus scaber</i> (ES-DCM) in human epithelial cancer cells. HeLa (a) and Caco-2 (b) cells were incubated with rhodamine 123 (Rho-123) in Dulbecco's modified eagle medium with 2% fetal bovine serum for 30 min at 37°C (accumulation phase), after which excess dye was washed and cells were re-incubated with vehicle control (dimethyl sulfoxide), verapamil, and ES-DCM for 60 min at 37°C (efflux phase). The mean cellular Rho-123 fluorescence in the efflux phase was analyzed by flow cytometry. Error bars represent ± standard error of the mean; <i>n</i> = 3, ***<i>P</i> < 0.001