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  Indian J Med Microbiol
 

Figure 2: Apoptotic effects of enriched fraction of Elephantopus scaber (ES-DCM) in human epithelial cancer cells. Cervical carcinoma (HeLa), lung adenocarcinoma (A549), breast carcinoma (MCF-7), and colon carcinoma (Caco2) cells were treated with the enriched fraction of ES (ES-DCM) for 48 h. Dimethyl sulfoxide and doxorubicin were used as vehicle control and positive control, respectively. At the end of the treatment, cells were harvested and stained to detect apoptosis using fluorescence microscopy. (a) Acridine orange/ethidium bromide staining to distinguish between necrosis and apoptosis. Uniformly stained green colored nuclei indicate live cells; condensed or fragmented nucleus with green or orange-red color indicates the apoptosis. (b) Hoechst 33342 staining to detect apoptosis. Condensed and fragmented nuclei indicate the hall mark properties of apoptosis. The data are representative of three independent experiments

Figure 2: Apoptotic effects of enriched fraction of <i>Elephantopus scaber</i> (ES-DCM) in human epithelial cancer cells. Cervical carcinoma (HeLa), lung adenocarcinoma (A549), breast carcinoma (MCF-7), and colon carcinoma (Caco2) cells were treated with the enriched fraction of ES (ES-DCM) for 48 h. Dimethyl sulfoxide and doxorubicin were used as vehicle control and positive control, respectively. At the end of the treatment, cells were harvested and stained to detect apoptosis using fluorescence microscopy. (a) Acridine orange/ethidium bromide staining to distinguish between necrosis and apoptosis. Uniformly stained green colored nuclei indicate live cells; condensed or fragmented nucleus with green or orange-red color indicates the apoptosis. (b) Hoechst 33342 staining to detect apoptosis. Condensed and fragmented nuclei indicate the hall mark properties of apoptosis. The data are representative of three independent experiments