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  Indian J Med Microbiol
 

Figure 2: Scatter plots indicative of apoptotic patterns for (a) MCF-7 cells untreated and treated with cotyledon extract, and (b) MDA-MB-231 cells untreated and treated with pericarp extract of Vatica diospyroides type SS fruit. Treatment dose levels were half 50% growth inhibition concentrations (IC50), IC50 and 2-fold IC50, for 24 h. The cells were stained with AnnexinV-FITC/PI and analyzed by flow cytometry. Viable, early apoptotic, late apoptotic, and nonviable cells are positioned in the lower left, lower right, upper right and upper left segments of the diagram. Yellow arrows indicate apoptotic progression of cells, whereas red arrow shows necrotic transition. Five thousand events were counted in each assay

Figure 2: Scatter plots indicative of apoptotic patterns for (a) MCF-7 cells untreated and treated with cotyledon extract, and (b) MDA-MB-231 cells untreated and treated with pericarp extract of <i>Vatica diospyroides</i> type SS fruit. Treatment dose levels were half 50% growth inhibition concentrations (IC<sub>50</sub>), IC<sub>50</sub> and 2-fold IC<sub>50</sub>, for 24 h. The cells were stained with AnnexinV-FITC/PI and analyzed by flow cytometry. Viable, early apoptotic, late apoptotic, and nonviable cells are positioned in the lower left, lower right, upper right and upper left segments of the diagram. Yellow arrows indicate apoptotic progression of cells, whereas red arrow shows necrotic transition. Five thousand events were counted in each assay