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  Indian J Med Microbiol
 

Figure 4: Quercetin treatment inhibits G6Pase activity and stimulated the phosphorylation of AMPK in H4IIE hepatocytes. H4IIE cells were treated with either vehicle (0.1% DMSO, 18 h) or quercetin (50 μM, 18 h). (a) G6Pase activity was assessed by measuring the rate of glucose formation in the presence of a non-limiting amount of G6P as described under "Materials and Methods." Insulin (100 nM, 18 h) served as the positive control. Results are expressed as mean % change ± SEM, relative to a vehicle-treated control group for three independent experiments of four to six replicates per condition. G6Pase activity data were normalized to total protein content per well. * denotes a significant difference (P ≤ 0.05). (b) Phosphorylation of AMPK was measured by western immunoblot. AICAR (2 mM) applied for 30 min served as the positive control. The upper immunoblot was probed with anti-phospho-AMPK and the lower blot was probed with β-actin as loading control. Blots shown are representative from 3 experiments

Figure 4: Quercetin treatment inhibits G6Pase activity and stimulated the phosphorylation of AMPK in H4IIE hepatocytes. H4IIE cells were treated with either vehicle (0.1% DMSO, 18 h) or quercetin (50 μM, 18 h). (a) G6Pase activity was assessed by measuring the rate of glucose formation in the presence of a non-limiting amount of G6P as described under P ≤ 0.05). (b) Phosphorylation of AMPK was measured by western immunoblot. AICAR (2 mM) applied for 30 min served as the positive control. The upper immunoblot was probed with anti-phospho-AMPK and the lower blot was probed with β-actin as loading control. Blots shown are representative from 3 experiments">