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  Indian J Med Microbiol
 

Figure 1: Quercetin increases glucose uptake and GLUT4 translocation in L6 GLUT4myc myotubes. Cells were treated with either 50 μM of quercetin, or with vehicle (0.1% DMSO) for 18 h. 100 nM insulin was applied for the last 15 min of the treatment in vehicle-treated cells. (a) Glucose uptake was assessed by the incorporation of 3H-deoxyglucose as described in Materials and Methods. Data are expressed relative to basal uptake observed in vehicle control treated cells (100%). Data represent the mean ± SEM of 3 experiments, each experiment composed of 3-4 replicates per condition. (b) GLUT4 translocation was assessed by measuring cell surface GLUT4myc using an enzyme-linked colorimetric assay, as described in Materials and Methods. Data are expressed relative to basal translocation observed in vehicle control treated cells (1.0). Data represent the mean SEM of 3 experiments, each experiment composed of 3-4 replicates per condition. * Indicates a significant (P ≤ 0.05) difference from the vehicle control group and indicates a significant (P ≤ 0.05) difference from insulin group as assessed by ANOVA

Figure 1: Quercetin increases glucose uptake and GLUT4 translocation in L6 GLUT4<i>myc</i> myotubes. Cells were treated with either 50 μM of quercetin, or with vehicle (0.1% DMSO) for 18 h. 100 nM insulin was applied for the last 15 min of the treatment in vehicle-treated cells. (a) Glucose uptake was assessed by the incorporation of 3H-deoxyglucose as described in Materials and Methods. Data are expressed relative to basal uptake observed in vehicle control treated cells (100%). Data represent the mean ± SEM of 3 experiments, each experiment composed of 3-4 replicates per condition. (b) GLUT4 translocation was assessed by measuring cell surface GLUT4myc using an enzyme-linked colorimetric assay, as described in Materials and Methods. Data are expressed relative to basal translocation observed in vehicle control treated cells (1.0). Data represent the mean  SEM of 3 experiments, each experiment composed of 3-4 replicates per condition. * Indicates a significant (<i>P</i> ≤ 0.05) difference from the vehicle control group and  indicates a significant (<i>P</i> ≤ 0.05) difference from insulin group as assessed by ANOVA