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  Indian J Med Microbiol
 

Figure 5: AMP-activated protein kinase activation by metformin enhances t-Bid expression, which is followed by cytochrome C release. (a) HSC-2 cells were treated with dimethyl sulfoxide (DMSO) or 2 mM of metformin for 24 h. Whole-cell lysates were analyzed by Western blot analysis using antibodies against t-Bid, Bak and Bad. *P < 0.05 compared with the DMSO-treated group; (b) The presence of cytochrome C in cytosolic fraction was detected by Western blot anlaysis. Cox4 and α-tubulin were used as fraction markers for the mitochondrial and cytosolic fraction, respectively. *P < 0.05 compared with the DMSO-treated group

Figure 5: AMP-activated protein kinase activation by metformin enhances t-Bid expression, which is followed by cytochrome C release. (a) HSC-2 cells were treated with dimethyl sulfoxide (DMSO) or 2 mM of metformin for 24 h. Whole-cell lysates were analyzed by Western blot analysis using antibodies against t-Bid, Bak and Bad. *<i>P</i> < 0.05 compared with the DMSO-treated group; (b) The presence of cytochrome C in cytosolic fraction was detected by Western blot anlaysis. Cox4 and α-tubulin were used as fraction markers for the mitochondrial and cytosolic fraction, respectively. *<i>P</i> < 0.05 compared with the DMSO-treated group