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  Indian J Med Microbiol
 

Figure 2: Effects of MEKS on the induction of apoptosis. (A) MDA-MB-231 cells were treated with various concentrations of MEKS for 4 h or with 30 nM paclitaxel for 24 h (positive control). Cells were stained with Annexin-V and 7-AAD and analysed by flow cytometry. Early apoptotic cells were stained by Annexin-V but not by 7-AAD (lower right quadrant, Q4), whereas late apoptotic cells are stained by both upper right quadrant, Q2.(B) Cells were treated with 0 ìg/ml (a), 3 ìg/ ml (b), 6 ìg/ml (c), 12 ìg/ml (d), or 25 ìg/ml (e) of MEKS, or 30 nM of paclitaxel for 24 h (f). Apoptosis was identified by Hoechst 33342 staining (Original Magnification, X400). The filled arrow indicates the presence of the characteristic morphological changes of apoptosis, including chromatin condensation and nuclear fragmentation

Figure 2: Effects of MEKS on the induction of apoptosis. (A) MDA-MB-231 cells were treated with various concentrations of MEKS for 4 h or with 30 nM paclitaxel for 24 h (positive control). Cells were stained with Annexin-V and 7-AAD and analysed by flow cytometry. Early apoptotic cells were stained by Annexin-V but not by 7-AAD (lower right quadrant, Q4), whereas late apoptotic cells are stained by both upper right quadrant, Q2.(B) Cells were treated with 0 ìg/ml (a), 3 ìg/ ml (b), 6 ìg/ml (c), 12 ìg/ml (d), or 25 ìg/ml (e) of MEKS, or 30 nM of paclitaxel for 24 h (f). Apoptosis was identified by Hoechst 33342 staining (Original Magnification, X400). The filled arrow indicates the presence of the characteristic morphological changes of apoptosis, including chromatin condensation and nuclear fragmentation