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  Indian J Med Microbiol
 

Figure 4: Mitochondrial effects of HE treatment. (a) Representative images of superoxide anions' negative and positive cells treated with 12.5 and 25 μg/ml of HE for 18 h, and 5 mm of hydrogen peroxide (positive control) for 30 min. B16F10-Nex2 cells were stained with 5 μM of DHE (dihydroethidium). Scale bar: 20 μm; (b) Representative images of mitochondrial ÄѰm following HE treatment. B16F10-Nex2 cells were treated with negative control and with 12 and 25 μg/ml of HE for 18 h (original magnification, 20×). Positive apoptotic cells were stained with annexin V (original magnification, 10X). (c) Percentage of TMRE (tetramethylrhodamine ethyl ester) and DHE positive cells; (d) Protective effect of N-acetyl cysteine (NAC) on HE-treated cells. B16F10-Nex2 cells (104) were pretreated for 2 h with 10 mM NAC, washed and incubated with 50 μg/ml of HE at 37°C for 18 h

Figure 4: Mitochondrial effects of HE treatment. (a) Representative images of superoxide anions' negative and positive cells treated with 12.5 and 25 μg/ml of HE for 18 h, and 5 mm of hydrogen peroxide (positive control) for 30 min. B16F10-Nex2 cells were stained with 5 μM of DHE (dihydroethidium). Scale bar: 20 μm; (b) Representative images of mitochondrial ÄѰm following HE treatment. B16F10-Nex2 cells were treated with negative control and with 12 and 25 μg/ml of HE for 18 h (original magnification, 20×). Positive apoptotic cells were stained with annexin V (original magnification, 10X). (c) Percentage of TMRE (tetramethylrhodamine ethyl ester) and DHE positive cells; (d) Protective effect of N-acetyl cysteine (NAC) on HE-treated cells. B16F10-Nex2 cells (104) were pretreated for 2 h with 10 mM NAC, washed and incubated with 50 μg/ml of HE at 37°C for 18 h