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  Indian J Med Microbiol
 

Figure 2: Morphological evidence and DNA degradation in HE-treated apoptotic melanoma cells. (a) B16F10-Nex2 cell morphology after treatment with 50 μg/ml HE for 18 h. Apoptotic bodies' formation is indicated by white arrows; (b) Evaluation of chromatin condensation in HE treated cells. B16F10-Nex2 cells (104) were treated with 50μg/ml of HE for 18 h, labeled with Hoechst dye, and analyzed by fluorescence microscopy. Arrows indicate pronounced chromatin condensation in treated cells (Magnification 60x) Scale bar: 20μm; (c) Agarose gel electrophoresis of DNA fragmentation in B16F10-Nex2 cells induced by 50 μg/ml HE treatment for 24 h; (d) Fluorescence microscopy for DNA fragmentation. Melanoma cells (5 × 104) were treated with 50 μg/ml of HE and 150 μM of combretastatin A4 as positive apoptotic control for 24 h, and DNA fragmentation was detected using a TUNEL assay (green fluorescence). DAPI (blue) were used for total cell nuclei (Scale bar: 20 μm)

Figure 2: Morphological evidence and DNA degradation in HE-treated apoptotic melanoma cells. (a) B16F10-Nex2 cell morphology after treatment with 50 μg/ml HE for 18 h. Apoptotic bodies' formation is indicated by white arrows; (b) Evaluation of chromatin condensation in HE treated cells. B16F10-Nex2 cells (104) were treated with 50μg/ml of HE for 18 h, labeled with Hoechst dye, and analyzed by fluorescence microscopy. Arrows indicate pronounced chromatin condensation in treated cells (Magnification 60x) Scale bar: 20μm; (c) Agarose gel electrophoresis of DNA fragmentation in B16F10-Nex2 cells induced by 50 μg/ml HE treatment for 24 h; (d) Fluorescence microscopy for DNA fragmentation. Melanoma cells (5 × 104) were treated with 50 μg/ml of HE and 150 μM of combretastatin A4 as positive apoptotic control for 24 h, and DNA fragmentation was detected using a TUNEL assay (green fluorescence). DAPI (blue) were used for total cell nuclei (Scale bar: 20 μm)