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  Indian J Med Microbiol
 

Figure 2: Effects of ST-WE on RANKL-induced MAP kinase activation. MAP kinase activation was represented by the levels of protein phosphorylation. RAW264.7 cells (2 × 105 cells/well in a 6-well plate) were pre-treated with or without ST-WE (400μg/ml) for 2 hours and then stimulated with RANKL (100ng/ml) for the time indicated. Western blot analysis was performed with whole cell lysates (10mg). Blots were probed with antibodies specific for MAP kinases. The densities of phosphorylated protein (p-) levels (upper panels) were normalized to the density of total protein levels (lower panels)

Figure 2: Effects of ST-WE on RANKL-induced MAP kinase activation. MAP kinase activation was represented by the levels of protein phosphorylation. RAW264.7 cells (2 × 105 cells/well in a 6-well plate) were pre-treated with or without ST-WE (400μg/ml) for 2 hours and then stimulated with RANKL (100ng/ml) for the time indicated. Western blot analysis was performed with whole cell lysates (10mg). Blots were probed with antibodies specific for MAP kinases. The densities of phosphorylated protein (p-) levels (upper panels) were normalized to the density of total protein levels (lower panels)