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  Indian J Med Microbiol
 

Figure 3: Assessment of apoptosis by Annexin-V/PI on the renal cell carcinoma (ACHN). The cells were treated with different concentrations of honey (2.5% and 20%) for 48 h (II and III, respectively) or media (control symbol I), and apoptosis was examined with flow- cytometry after Annexin-V/PI double staining. The necrotic cells lost cell membrane integrity that permits PI entry. Viable cells exhibit Annexin-V/PI; early apoptotic cells exhibit Annexin-V/PI −; and late apoptotic cells or necrotic cells exhibit Annexin-V+/PI+

Figure 3: Assessment of apoptosis by Annexin-V/PI on the renal cell carcinoma (ACHN). The cells were treated with different concentrations of honey (2.5% and 20%) for 48 h (II and III, respectively) or media (control symbol I), and apoptosis was examined with flow- cytometry after Annexin-V/PI double staining. The necrotic cells lost cell membrane integrity that permits PI entry. Viable cells exhibit Annexin-V/PI; early apoptotic cells exhibit Annexin-V/PI −; and late apoptotic cells or necrotic cells exhibit Annexin-V+/PI+