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  Indian J Med Microbiol
 

Figure 3 :A percentage of 0.8% agarose gel electrophoresis of the plasmid (resistance plasmid) treated with essential oil. Aliquots of R-plasmid DNA (5 l) were incubated with 5 l (35 g/ml) of the essential oil for 45 minutes at 37C. After incubation, the reaction mixtures were submitted to agarose gel electrophoresis. Lane 1: Plasmid extracted from isolate 1 plus essential oil. Lane 2: Plasmid extracted from isolate 2 plus essential oil. I = supercoiled form (SC); II = open circle form (OC), and III = linear form (L). (Reproduction size at column width)

Figure 3 :A percentage of 0.8% agarose gel electrophoresis of the plasmid (resistance plasmid) treated with essential oil. Aliquots of R-plasmid DNA (5 l) were incubated with 5 l (35 g/ml) of the essential oil for 45 minutes at 37C. After incubation, the reaction mixtures were submitted to agarose gel electrophoresis. Lane 1: Plasmid extracted from isolate 1 plus essential oil. Lane 2: Plasmid extracted from isolate 2 plus essential oil. I = supercoiled form (SC); II = open circle form (OC), and III = linear form (L). (Reproduction size at column width)