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Table of Contents
January 2014
Volume 10 | Issue 1 (Supplement)
Page Nos. 1-205
Online since Friday, February 21, 2014
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ORIGINAL ARTICLES
Effect of different growth stages of
Ziziphora clinopodioides
Lam. on its chemical composition
p. 1
Wenhuan Ding, Tao Yang, Feng Liu, Shuge Tian
DOI
:10.4103/0973-1296.127329
PMID
:24914287
Aims:
The aim of this study is to monitor the changes in the chemical composition of
Ziziphora clinopodioides
Lam. throughout nine different growth stages.
Materials and Methods:
Volatile components such as essential oils were analyzed using the gas chromatography (GC) and GC-mass spectrometry, and the contents of non-volatile components were determined by a visible spectrophotometer.
Results:
Hydro-distilled essential oil content ranged from a minimum of 1.1% (in the post-flowering stage) to a maximum of 1.8% (in the flowering stage). The essential oils included pulegone, which was the most abundant component (77.48-87.3%), p-menthanone (2.79-12.39%), trans-isopulegone (1.04-2.06%), d-limonene (0.51-3.03%) and eucarvone (1.5-4.48%). The contents of non-volatile components, such as that of total phenolics (TPC), total flavonoids (TFC), total triterpenoids content (TTC) and total free amino acids content (TFAAC) were measured using visible spectrophotometry. In the growing stage, TPC, TFC, TTC and TFAAC were 9.91-12.80 mg/g, 29.84-50.63 mg/g, 0.57-1.41 mg/g and 13.33-28.56 mg/g, respectively.
Conclusion:
These data can be used as a basis to determine the optimal harvest time of
Z. clinopodioide
Lam.
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Antimicrobial, antibiofilm and cytotoxic activities of
Hakea sericea
Schrader extracts
p. 6
Ângelo Luís, Luiza Breitenfeld, Susana Ferreira, Ana Paula Duarte, Fernanda Domingues
DOI
:10.4103/0973-1296.127331
PMID
:24914310
Background:
Hakea sericea
Schrader is an invasive shrub in Portuguese forests.
Objective:
The goal of this work was to evaluate the antimicrobial activity of
H. sericea
extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of these extracts, against human cells, were assessed.
Materials and
Methods:
The antimicrobial activity of the methanolic extracts of
H. sericea
was assessed by disk diffusion assay and Minimum Inhibitory Concentration (MIC) value determination. The antibiofilm activity was determined by quantification of total biofilm biomass with crystal violet. Cytotoxicity was evaluated by hemolysis assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test.
Results:
For Gram-positive bacteria, MIC values of
H. sericea
methanolic extracts ranged between 0.040 and 0.625 mg/mL, whereas the fruits extract yielded the lowest MIC for several strains of microorganisms, namely,
S. aureus
,
B. cereus
,
L. monocytogenes
and clinical methicillin-resistant
S. aureus
(MRSA). Stems and fruits extract at 2.5 mg/mL effectively eradicated the biofilm of
S. aureus
ATCC 25923, SA 01/10 and MRSA 12/10. Regarding leaves extract, hemolysis was not observed, and in the case of stems and fruits, hemolysis was verified only for higher concentrations, suggesting its low toxicity. Fruits extract presented no toxic effect to normal human dermal fibroblasts (NHDF) cells however for concentrations of 0.017 and 0.008 mg/mL this extract was able to decrease human breast adenocarcinoma cells (MCF-7) viability in about 60%, as MTT test results had confirmed. This is a clearly demonstrator of the cytotoxicity of this extract against MCF-7 cells.
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An enzoinformatics study targeting polo-like kinases-1 enzyme: Comparative assessment of anticancer potential of compounds isolated from leaves of
Ageratum houstonianum
p. 14
Syed Mohd Danish Rizvi, Shazi Shakil, Mohd Zeeshan, Mohd Sajid Khan, Sibhghatulla Shaikh, Deboshree Biswas, Adnan Ahmad, Mohammad Amjad Kamal
DOI
:10.4103/0973-1296.127333
PMID
:24914294
Natural products from plant sources, embracing inherently ample structural diversity than synthetic ones are the major sources of anticancer agents and will constantly play as protagonists for discovering new drugs. Polo-like kinases (PLKs) play a leading role in the ordered execution of mitotic events and 4 mammalian PLK family members have been identified. PLK1 is an attractive target for anticancer drugs in mammalian cells, among the four members of PLKs. The present study expresses the molecular interaction of compounds (1,2-Benzenedicarboxylic acid bis (2 ethylhexyl) ester, squalene, 3,5-bis (1,1-dimethylethyl) phenol, Pentamethyl tetrahydro-5H-chromene, (1,4-Cyclohexylphenyl) ethanone and 6-Vinyl-7-methoxy-2,2-dimethylchromene) isolated from methanolic extract of leaves of
Ageratum houstonianum
with PLK1 enzyme. Docking between PLK1 and each of these compounds (separately) was performed using "Auto dock 4.2." (1,4-Cyclohexylphenyl) ethanone showed the maximum potential as a promising inhibitor of PLK1 enzyme with reference to ∆G (−6.84 kcal/mol) and Ki (9.77 μM) values. This was sequentially followed by Pentamethyl tetrahydro-5H-chromene (∆G = −6.60 kcal/mol; Ki = 14.58 μM), squalene (∆G = −6.17 kcal/mol; Ki = 30.12 μM), 6-Vinyl-7-methoxy-2,2-dimethylchromene (∆G = −5.91 kcal/mol; Ki = 46.68 μM), 3, 5-bis (1,1-dimethylethyl) phenol (∆G = −5.70 kcal/mol; Ki = 66.68 μM) and 1,2-Benzenedicarboxylic acid bis (2 ethylhexyl) ester (∆G = −5.58 kcal/mol; Ki = 80.80 μM). These results suggest that (1,4-Cyclohexylphenyl) ethanone might be a potent PLK1 inhibitor. Further,
in vitro
and
in vivo
rumination are warranted to validate the anticancer potential of (1,4-Cyclohexylphenyl) ethanone.
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Simultaneous determination of five major compounds in the traditional medicine Pyeongwee-San by high performance liquid chromatography-diode array detection and liquid chromatography-mass spectrometry/mass spectrometry
p. 22
Bohyoung Lee, Jin Bae Weon, Bo-Ra Yun, Jiwoo Lee, Min Rye Eom, Choong Je Ma
DOI
:10.4103/0973-1296.127335
PMID
:24914305
Background:
Pyeongwee-San (PWS) has been widely used for treating acute gastritis, chronic, and gastritis.
Objective:
In this paper, simultaneous determination of five compounds (naringin, hesperidin, glycyrrhizin, atractylenolide III, and magnolol) from traditional medicine PWS using the high performance liquid chromatography (HPLC) was established for quality control.
Materials
and
Methods:
Optimum separations were obtained with a SHISEIDO C18 reverse-phase column by gradient elution with 0.1% Trifluoroacetic acid (TFA) water-acetonitrile as the mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 205 nm and 250 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test.
Results:
The calibration curves were linear over the established range with R
2
> 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.09 to 0.43 and 0.27 to 1.29 μg/mL. The method exhibited intra-day and inter-day precision range between 0.01-1.86% and 0.04-0.35% respectively. The recoveries of five compounds in PWS were in the range between 93.18-106.40%, and 0.20-1.51%. The application of this method was identified through the successful analysis of five compounds in 12 batches of PWS. In addition, identification of five compounds was confirmed by a liquid chromatography method and mass spectrometry.
Conclusion:
The HPLC method was could be accomplished to the quality control and stable experiment for the preparations consisted of five major compounds.
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Element analysis and characteristic identification of non-fumigated and sulfur-fumigated
Fritillaria thunbergii Miq.
using microwave digestion-inductively coupled plasma atomic emission spectrometry combined with Fourier transform infrared spectrometry
p. 30
Yajing Lou, Hao Cai, Xiao Liu, Sicong Tu, Ke Pei, Yingying Zhao, Gang Cao, Songlin Li, Kunming Qin, Baochang Cai
DOI
:10.4103/0973-1296.127337
PMID
:24914306
Background:
Sulfur-fumigation may induce chemical transformation of traditional Chinese medicines leading to harmful effects following patient ingestion. For quality control, it is urgently needed to develop a reliable and efficient method for sulfur-fumigation identification.
Materials and Methods:
The spectrochemical identification of non-fumigated and sulfur-fumigated
Fritillaria thunbergii Miq.
was carried out to evaluate inorganic elements and organic components. The concentrations of 12 elements, including Zn, Mn, Cu, Fe, Li, Mg, Sr, Pb, As, Cd, Hg, and S of samples were determined by microwave digestion - inductively coupled plasma atomic emission spectrometry (ICP-AES). Meanwhile, Fourier transform infrared spectrometry (FTIR) was used for the study of chemical group characteristic reactions after sulfur-fumigation.
Results:
The concentrations of Fe, Mg, Hg, and S elements showed significant differences between non-fumigated and sulfur-fumigated Fritillaria thunbergii Miq. The characteristic stretching vibrations of some groups in FTIR spectra, such as -OH, -S = O and -S-O, provided the identification basis for the discrimination of non-fumigated and sulfur-fumigated Fritillaria thunbergii Miq.
Conclusion:
The application of microwave digestion - ICP-AES was successfully used in combination with FTIR to authenticate and evaluate the quality of medicinal
Fritillaria thunbergii Miq.
Further applications of this technique should be explored.
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Gas chromatography-mass spectroscopy analysis of bioactive petalostigma extracts: Toxicity, antibacterial and antiviral activities
p. 37
FR Kalt, IE Cock
DOI
:10.4103/0973-1296.127338
PMID
:24914307
Background:
Petalostigma pubescens
and
Petalostigma triloculare
were common components of pharmacopeia's of multiple Australian Aboriginal tribal groupings which traditionally inhabited the areas in which they grow. Among these groups, they had a myriad of medicinal uses in treating a wide variety of bacterial, fungal and viral infections. This study was undertaken to test
P. pubescens
and
P. triloculare
leaf and fruit extracts for the ability to inhibit bacterial and viral growth and thus validate Australian Aboriginal usage of these plants in treating bacterial and fungal diseases.
Materials and Methods:
P. pubescens
, and
P. triloculare
leaves and fruit were extracted and tested for antimicrobial, antiviral activity and toxicity. The bioactive extracts were further examined by RP-HPLC and GC-MS to identify the component compounds.
Results:
The methanol, water and ethyl acetate leaf and fruit extracts of displayed potent antibacterial activity. The methanol and ethyl acetate extracts displayed the broadest specificity, inhibiting the growth of 10 of the 14 bacteria tested (71%) for the leaf extract and 9 of the 14 bacteria tested (64%) for the fruit extracts. The water extracts also had broad spectrum antibacterial activity, inhibiting the growth of 8 (57%) and 7 (50%) of the 14 bacteria tested, respectively. All antibacterial extracts were approximately equally effective against Gram-positive and Gram-negative bacteria, inhibiting the growth of 50-75% of the bacteria tested. The methanol, water and ethyl acetate extracts also displayed antiviral activity in the MS2 plaque reduction assay. The methanol and water extracts inhibited 26.6-49.0% and 85.4-97.2% of MS2 plaque formation, respectively, with the fruit extracts being more potent inhibitors. All ethyl acetate extracts inhibited 100% of MS2 plaque formation. All extracts were also non-toxic or of low toxicity. Analysis of these extracts by RP-HPLC showed that the
P. triloculare
ethyl acetate fruit extract was the least complex of the bioactive extracts. Subsequent analysis of this extract by GC-MS revealed that it contained 9 main compounds: acetic acid; 2,2-dimethoxybutane; 4-methyl-1,3-dioxane; decane; unadecane; 2-furanmethanol; 1,2-benzenediol; 1,2,3-benzenetriol; and benzoic acid.
Conclusion:
These studies validate Australian Aboriginal therapeutic usage of
Petalostigma
species and indicate their medicinal potential.
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Antidepressant-like activity of Chaihu-Shugan-San aqueous extract in rats and its possible mechanism
p. 50
Sue Wang, Suiyu Hu, Chunhu Zhang, Juan Qiu, Yunhui Li
DOI
:10.4103/0973-1296.127342
PMID
:24914308
Background:
Chaihu-Shugan-San (CHSGS), a traditional Chinese medicinal formula, is commonly used for the treatment of depression in China. However, the molecular mechanism underlying its antidepressant action is unknown.
Objective:
The objective of this study is to evaluate the antidepressant-like effects of CHSGS and further explore the possible molecular mechanism implicated in its actions.
Materials and Methods:
The rats were randomly divided into four groups: The normal control group, the model control group, the CHSGS group and the fluoxetine control group. The antidepressant-like effects of CHSGS aqueous extract were assessed in rats exposed to chronic mild stress (CMS) using the open-field test and sucrose water consumption test, its underlying mechanism of anti-depression was explored by determining the effect of CHSGS on the extracellular signal-regulated kinase (ERK) and phospho-ERK (P-ERK) in the hippocampus using western blot. The aqueous extract of CHSGS at a dose of standard (5.9 g/kg·d) was administered intragastrically for 14 days during the CMS model while the fluoxetine control group was given at the same time using fluoxetine hydrochloride (1.8 mg/kg·d).
Results:
The stressed rats demonstrated decreased locomotor activity in open field test and reduction in sucrose consumption and decreased levels of P-ERK1/2 and the ratio of P-ERK1/2 to total ERK1/2 in the hippocampus. CHSGS alleviated the depressive-like behaviors and increased levels of P-ERK1/2 and the ratio of P-ERK1/2 to total ERK1/2 in stressed rats as well as fluoxetine.
Conclusion:
In summary, these results suggest that CHSGS aqueous extract possesses an antidepressant-like activity in CMS induced depression model rats, which might be mediated, at least in part, by reversing the stress-induced disruption of ERK activity.
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Optimization of quercitrin and total flavonoids extraction from Herba Polygoni Capitati by response surface methodology
p. 57
Fengwei Ma, Yang Zhao, Xiaojian Gong, Yu Xie, Xin Zhou
DOI
:10.4103/0973-1296.127343
PMID
:24914309
Objective:
To optimize the conditions for extraction of quercitrin and total flavonoids (TF) from Herba Polygoni Capitati (Touhualiao in Chinese) by using response surface methodology (RSM).
Materials and Methods:
A central composite design (CCD) was adopted to investigate the effects of three independent variables including solvent composition (%), solvent-material ratio (ml/g) and extraction time (min) on the responses, quercitrin and TF yields.
Results:
The optimized conditions of the extraction are as follows: Ethanol concentration, 65.63%; solvent-material ratio, 10.55:1 (ml/g); extraction time, 54.33 min. The established mathematical model described the factors of experimental parameters well and provided a statistically accurate prediction of the optimum yields of quercitrin and TF.
Conclusion:
The experimental values agreed with those predicted by the established mathematical model, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions.
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In vitro
and
in silico
studies on the anticancer and apoptosis-inducing activities of the sterols identified from the soft coral,
subergorgia reticulata
p. 65
Kuniyil Byju, Vattoni Anuradha, Gopalakrishnapai Vasundhara, S Muraleedharan Nair, N Chandramohana Kumar
DOI
:10.4103/0973-1296.127345
PMID
:24914311
Background:
Gorgonians and other octocorals are known to possess a huge array of secondary metabolites in which sterols are the major group of secondary metabolites apart from sesquiterpenes and diterpenes, and the bioactive metabolites could show marked biomedical potential for future drug discovery.
Objective
: This study was intended for the isolation and identification of sterols from the octocoral
Subergorgia reticulata
and to evaluate the anticancer and apoptosis-inducing activities of the identified sterols through
in vitro
and
in silico
approach.
Materials and Methods
: The organism was collected from Lakshadweep Island. The isolated sterols were identified using Gas chromatography-mass spectrometry (GC-MS). The structure was confirmed by using comparison of their spectra those in National Institute of Standard Technology (NIST) library. The apoptosis inducing effect of identified sterols were determined by PASS online prediction.
In vitro
cytotoxity studies were carried out using Dalton's lymphoma ascites cells (DLA) and the cell viability was determined by trypan blue exclusion method.
Results
: Six sterols were identified from the soft coral
S. reticulata.
They are Cholesta-5,22-diene-3ol (3β), Ergosta-5-22-dien-3ol (3β,22E 24S), Cholesterol, 26,26-Dimethyl-5,24(28)-ergostadien-3β-ol. β-sitosterol, and Fucosterol.
In silico
predictions showed that the identified sterols exhibited remarkable apoptosis agonist activity. The probability of apoptosis agonist activity were found maximum for 26,26-Dimethyl-5,24 (28)-
S. reticulata
sterol fractions isolated were found to be having anticancer activity.
Conclusions
: These findings suggest that
S. reticulata
contained biologically active sterol compounds that may be useful in the treatment of cancer.
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Simultaneous quantification of nine major active components in traditional Chinese prescription Mahuang decoction and the influence of herbal compatibility on their contents
p. 72
Yu He, Ying Zhu, Ruping Zhang, Lijun Ge, Haitong Wan
DOI
:10.4103/0973-1296.127346
PMID
:24914312
Background:
Mahuang decoction (MHD), a famous classic traditional Chinese formula, has been extensively applied for treating cold, influenza, asthma, acute bronchitis, and other pulmonary diseases. However, the interaction among four drugs of MHD has not been clearly deciphered from the aspect of molecular composition.
Objective:
To assess the quality of MHD and explore the interplay among different prescription drugs.
Materials and Methods:
A reversed-phase high performance liquid chromatography (RP-HPLC) coupled with diode array detector (DAD) method for the simultaneous separation and determination of nine bioactive components was developed. A somatomedin A (SMA)-phenyl column (4.6 mm Χ 250 mm, 5 μm) was eluted by a gradient mobile phase contained acetonitrile and 0.05% formic acid-0.05% triethylamine aqueous solution. Four detection wavelengths (210, 252, 278, and 291 nm) were utilized for the quantitative analysis due to the different ultraviolet (UV) spectra of these compounds.
Results:
Satisfactory separation was obtained for all the components, and the assay was fully validated in respects of linearity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R
2
≥ 0.9991) within the test ranges. The relative standard deviations (RSDs) for intra- and interday repeatability were not more than 1.70 and 2.66%, respectively. The spike recoveries of nine components varied from 97.50 ± 1.69 to 99.27 ± 1.37%.
Conclusion:
The established method was successfully applied to analyze nine active compounds in decoction samples of various drug compatibilities of MHD. The variations of contents were obvious for different combinations, which hinted the mutual promotion or inhibition of componential dissolution among four herbs of MHD.
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Antiasthmatic effects of schizandrae fructus extract in mice with asthma
p. 80
Hyungwoo Kim, Yong-Tae Ahn, Youn Sook Kim, Su In Cho, Won Gun An
DOI
:10.4103/0973-1296.127348
PMID
:24914313
Background:
Schizandrae fructus (SF), the fruit of
Schisandra chinensis
, has been used for the treatment of cough, wheezing, dry mouth, hepatitis, cardiovascular disease, and as a tonic and astringent in China, Japan, and Korea.
Objective:
Investigation of the antiasthmatic effects of SF.
Materials and Methods:
We investigated the effects of SF on airway hyperresponsiveness (AHR) to methacholine, production levels of antigen-specific antibodies, and histopathological changes in the lung tissue in a mouse model (Balb/c) of asthma induced by repeated intranasal instillation of an antigen.
Results:
SF lowered AHR to methacholine (
P
< 0.05), antigen-specific immunoglobulin E (IgE) level (
P
< 0.01), and immune cell infiltration in mice with asthma. Prednisolone (PD) effectively decreased AHR (
P
< 0.01), total antibody (
P
< 0.01) and IgE (
P
< 0.01) levels, and immune cell infiltration. SF and PD did not affect the levels of antigen-specific IgG1 and IgG2a antibodies.
Conclusion:
Our data suggest that SF has possible application as an antiasthmatic drug. We also suggest that SF could be used as a complementary or alternative medicine to glucocorticoids.
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Assessment of potency of PC-complexed
Ocimum sanctum
methanol extract in embryonated eggs against Influenza virus (H1N1)
p. 86
Priyanka Jadhav, Hingorani Lal, Nilima Kshirsagar
DOI
:10.4103/0973-1296.127352
PMID
:24914314
Background:
Despite of new vaccines, the threat of influenza infection persists. In addition, availability, cost, duration of protection rendered and effectiveness of vaccines additional to the need of effective drug therapy makes influenza a challenge, which the globe faces. Traditionally used herbs and their decoctions are used for ages to cure symptoms similar to influenza.
Tulsi
or
Ocimum sanctum
is one of these major herbs used for influenza-like disease treatment. We attempted to explore a new methodology for assessing phosphatidyl choline (PC)-complexed
O. sanctum
methanol extract in embryonated vaccine quality eggs model.
Materials and Methods:
The PC-complexed
O. sanctum
methanol extract was prepared and standardized using High-Performance Liquid Chromatography (HPLC). (Data not provided here) Nine to 11 days embryonated eggs were inoculated with the virus and drug mixture and then harvested to perform a hemagglutination (HA) test on the allantoic fluid. The experiments were performed at three different concentrations of ursolic acid with various virus concentration and dose levels of drugs. The HA titer was calculated from all experiments and observed for any inhibition of virus
.
Results:
In initial experiments, matrix method for drug and virus concentration was employed. It was observed that the drug exhibited some response for 3log EID
50
(egg infective dose) in few samples at 1:2 HA titer, but no response was observed at 4log EID
50
. In subsequent experiment, all the virus titers from 7log EID
50
to 2log EID
50
demonstrated positive HA titer of 1:64. However, the drug failed to exhibit any significant inhibition at any level of demonstrable virus titer. At all the concentrations,
O. sanctum
extracts were found to be safe.
Conclusion:
The embryonated egg model may be utilized further to screen other drugs, which possess direct inhibitory properties like neuraminidase inhibition, and
O. sanctum
does not inhibit the influenza virus in this model at the given concentration.
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Silybum marianum
oil attenuates oxidative stress and ameliorates mitochondrial dysfunction in mice treated with D-galactose
p. 92
Shu Yun Zhu, Ying Dong, Jie Tu, Yue Zhou, Xing Hua Zhou, Bin Xu
DOI
:10.4103/0973-1296.127353
PMID
:24914315
Background:
Silybum
marianum
has been used as herbal medicine for the treatment of liver disease, liver cirrhosis, and to prevent liver cancer in Europe and Asia since ancient times.
Silybum
marianum
oil (SMO), a by-product of silymarin production, is rich in essential fatty acids, phospholipids, sterols, and vitamin E. However, it has not been very good development and use.
Objective:
In the present study, we used olive oil as a control to investigate the antioxidant and anti-aging effect of SMO in D-galactose (D-gal)-induced aging mice.
Materials
and
Methods:
D-gal was injected intraperitoneally (500 mg/kg body weight daily) for 7 weeks while SMO was simultaneously administered orally. The triglycerides (TRIG) and cholesterol (CHOL) levels were estimated in the serum. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), monoamine oxidase (MAO), malondialdehyde (MDA), caspase-3, and Bcl-2 were determined in the liver and brain. The activities of Na
+
-K
+
-adenosine triphosphatase (ATPase), Ca
2+
-Mg
2+
-ATPase, membrane potential (ΔΨm), and membrane fluidity of the liver mitochondrial were estimated.
Results:
SMO decreased levels of TRIG and CHOL in aging mice. SMO administration elevated the activities of SOD, GSH-Px, and T-AOC, which are suppressed by aging. The levels of MAO and MDA in the liver and brain were reduced by SMO administration in aging mice. Enzyme linked immunosorbent assay showed that SMO significantly decreased the concentration of caspase-3 and improved the activity of Bcl-2 in the liver and brain of aging mice. Furthermore, SMO significantly attenuated the D-gal induced liver mitochondrial dysfunction by improving the activities of Na
+
-K
+
-ATPase, Ca
2+
-Mg
2+
-ATPase, membrane potential (ΔΨm), and membrane fluidity.
Conclusion:
These results indicate that SMO effectively attenuated oxidative damage and improved apoptosis related factors as well as liver mitochondrial dysfunction in aging mice.
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Chemical analysis, antioxidant, antichemotactic and monoamine oxidase inhibition effects of some pteridophytes from Brazil
p. 100
Juliana MM Andrade, Carolina dos S Passos, Roger R Dresch, Maria Angélica Kieling-Rubio, Paulo Roberto H Moreno, Amélia T Henriques
DOI
:10.4103/0973-1296.127354
PMID
:24914288
Background:
Ferns are a group of plants that have been little explored from a chemical and biological perspective but that have interesting potential, occurring in various parts of the world.
Objective:
This work investigates the chemical profile and the biological effects of ferns from Brazil.
Materials and Methods:
Analyses were performed using rapid performance liquid chromatography (RP-LC) with a diode array detector (DAD). Extracts were tested for their
in vitro
antioxidant activity, by the total reactive antioxidant potential method and for their antichemotactic potential, by the Boyden chamber method. Cytotoxic effects were assessed by lactate dehydrogenase levels, while the monoamine oxidase (MAO) assay was carried out using a fluorescence-based method.
Results:
Different chemical compositions were found for the studied ferns, such as
Asplenium gastonis
, in which hesperidin was identified in its extract, while
A. serra
showed the presence of xanthone mangiferin. The most samples with highest antioxidant activity were the
Asplenium serra
,
Lastreopsis amplissima
and
Cyathea dichromatolepis
extracts, at 10 μg/mL. High antichemotactic activity was found for
A. serra
(94.06%) and
Didymochlaena truncatula
(93.41%), at 10 μg/mL. The extracts showed no cytotoxicity at the highest concentration. Against MAO-A,
D. truncatula
(82.61%),
Alsophila setosa
(82.21%),
Cyathea phalerata
(74.07%) and
C. delgadii
(70.32%) were the most active extracts (100 μg/mL).
Conclusion:
The hypothesis was considered that phenolics and triterpenes are responsible for these pronounced activities.
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Stereo and region-selective biosynthesis of two new dihydroartemisinic acid glycosides by suspension-cultured cells of
Artemisia annua
p. 110
Jianhua Zhu, Zihan Zeng, Liyan Song, Yanshan Hu, Wei Wen, Rongming Yu
DOI
:10.4103/0973-1296.127357
PMID
:24914289
Background:
The system of plant-cultured cells is one of the optimal systems to investigate biosynthesis pathway and their bioactive intermediates.
Objective:
To study the biosynthesis of dihydroartemisinic acid (1) by suspension-cultured cells of
Artemisia annua
.
Materials and Methods:
Substrate (compound 1) was administered into the suspension-cultured cells of
A. annua
and co-cultured for 2 days. The methanol extract was separated on various column chromatography methods and the structures of two biosynthesis products were elucidated based on the analysis of
1
H NMR,
13
C NMR, 2D NMR, and ESI-MS. Time-course curve was also established. Furthermore, in vitro antitumor activities of compounds 1-3 against HepG2, K562, and A549 cell lines were evaluated by MTT assay.
Results:
Two new compounds were obtained, namely 3α-hydroxy-dihydroartemisinic acid-α-D
-
glucopyranosyl ester (2) and 15-hydroxy-cadin-4-en-12-oic acid-β-d
-
glucopyranosyl ester (3). The results demonstrated that the cultured cells of A. annua possessed the abilities to stereo-selective hydroxylate and region-selective glycosylate sesquiterpene compounds in a highly efficient manner. Inhibitory effects of compounds 1-3 on proliferation of HepG2, K562, and A549 cell lines
in vitro
were also investigated.
Conclusion:
Two new dihydroartemisinic acid glycosides were obtained by stereo- and region-selective biosynthesis with cultured cells of
A. annua
.
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Comparison of pharmacokinetic behavior of two iridoid glycosides in rat plasma after oral administration of crude
Cornus officinals
and its jiuzhipin by high performance liquid chromatography triple quadrupole mass spectrometry combined with multiple reactions monitoring mode
p. 115
Xiaocheng Chen, Gang Cao, Jianping Jiang
DOI
:10.4103/0973-1296.127358
PMID
:24914290
Objective:
The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed
Cornus
officinals.
Materials and Methods:
A rapid, selective and specific high-performance liquid chromatography/electrospray ionization tandem mass spectrometry with multiple reactions monitoring mode was developed to simultaneously investigate the pharmacokinetic profiles of morroniside and loganin in rat plasma after oral administration of crude
C.
officinals
and its jiuzhipin.
Results:
The morroniside and loganin in crude and processed
C.
officinals
could be simultaneously determined within 7.4 min. Linear calibration curves were obtained over the concentration ranges of 45.45-4800 ng/mL for all the analytes. The intra-and inter-day precisions relative standard deviation was lesser than 2.84% and 4.12%, respectively.
Conclusion:
The pharmacokinetic parameters of two iridoid glucosides were also compared systematically between crude and processed
C.
officinals
. This paper provides the theoretical proofs for further explaining the processing mechanism of Traditional Chinese Medicines.
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Characteristics and kinetics of catalpol degradation and the effect of its degradation products on free radical scavenging
p. 122
Guo-dong Wei, Xue-sen Wen
DOI
:10.4103/0973-1296.127360
PMID
:24914291
Background:
The dried and steamed roots of
Rehmannia glutinosa
have different pharmacological functions and indications. Catalpol, the main active component of the dried root, was found to be entirely degraded together with amino acids and some oligosaccharides during preparation of the steamed root. Its degradation may contribute to the differences between dried and steamed roots.
Objective:
To reveal the characteristics and kinetics of catalpol degradation, and evaluate its influence on the antioxidant properties of steamed
Rehmannia
roots.
Materials
and
Methods:
Purified catalpol was heated under different pH and temperature values for different times, alone or with sugars or amino acids. Catalpol concentration was determined by high-performance liquid chromatography. Browning was expressed by the absorbance at 420 nm (A
420
), and antioxidation was displayed by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging ability (SA
DPPH
). Activation energy was calculated using Arrhenius plotting.
Results:
Catalpol was stable in neutral conditions and sensitive to acidic pH under high temperatures. Sugars had no influence on catalpol degradation; however, most amino acids, except for proline, could promote the degradation, and were associated with an increase in A
420
and SA
DPPH
values. These changes were proved to be mainly related with catalpol aglycone and were dependent on the presence of amino acids. Catalpol degradation was found to obey first-order kinetics. The activation energies were 81.7, 88.8 and 98.7 kJ/mol at pH 4.0, 5.0, and 6.0 respectively, and 70.7 kJ/mol at pH 4.0 value and in the presence of glycine.
Conclusions:
Catalpol degradation, especially, in the presence of amino acids can substantially boost antioxidant properties of the products; therefore, the traditional method for processing
Rehmannia
root seems rather apt.
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Composition of the essential oil of
Cynanchum mongolicum
(Asclepiadaceae) and insecticidal activities against
Aphis glycines
(Hemiptera: Aphidiae)
p. 130
Wang Yang, An Zhao, Zhen Congai, Liu Qizhi, Shi Wangpeng
DOI
:10.4103/0973-1296.127362
PMID
:24914292
Background:
Farmers have applied
Cynanchum mongolicum
(Maxim) to control crop pests. The aim of this study was to analyze composition of essential oil from
C. mongolicum
, evaluate insecticidal activities against
Aphis glycines
, and lethal doses.
Materials and Methods:
Essential oil from
C. mongolicum
was efficiently extracted by steam distillation. The main components of the oil were analyzed with a gas chromatography/mass spectrometry (GC/MS) system, and the insecticidal activity of the essential oil on soybean aphids
Aphis glycines
was tested using a variety of methods.
Results:
The components of the essential oil from
C. mongolicum
mainly included terpenes and ester compounds, of which (Z)-3-Hexen-1-ol acetate, cis-3-hexenyl isovalerate, α-farnesene, and β-caryophyllene accounted for 15.8, 10.4, 8.4, and 5.5%, respectively. With 1- and 2-day exposure, the essential oil showed pronounced contact toxicity (median lethal concentration (LC
50
) =37.8 and 38.4 μL/mL, respectively), weak fumigant toxicity (LC
50
= 139.7 and 139.9 μL/L, respectively). The essential oil showed strong deterrent activity on soybean aphids in 2 and 4 h.
Conclusion:
The essential oil of
C. mongolicum
contained insecticidal components and possessed contact toxicity and deterrent activity to
A. glycine
s.
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Identification of sulfur fumed
Pinelliae Rhizoma
using an electronic nose
p. 135
Xia Zhou, Jun Wan, Liang Chu, Wengang Liu, Yafeng Jing, Chunjie Wu
DOI
:10.4103/0973-1296.127363
PMID
:24914293
Background:
Pinelliae Rhizoma
is a commonly used Chinese herb which will change brown during the natural drying process. However, sulfur fumed
Pinelliae Rhizoma
will get a better appearance than naturally dried one. Sulfur fumed
Pinelliae Rhizoma
is potentially toxical due to sulfur dioxide and sulfites formed during the fuming procedures. The odor components in sulfur fumed
Pinelliae Rhizoma
is complex. At present, there is no analytical method available to determine sulfur fumed
Pinelliae Rhizoma
simply and rapidly. To ensure medication safety, it is highly desirable to have an effective and simple method to identify sulfur fumed
Pinelliae Rhizoma
.
Materials and Methods:
This paper presents a novel approach using an electronic nose based on metal oxide sensors to identify whether
Pinelliae Rhizoma
was fumed with sulfur, and to predict the fuming degree of
Pinelliae Rhizoma
. Multivariate statistical methods such as principal components analysis (PCA), discriminant factorial analysis (DFA) and partial least squares (PLS) were used for data analyzing and identification. The use of the electronic nose to discriminate between different fuming degrees
Pinelliae Rhizoma
and naturally dried
Pinelliae Rhizoma
was demonstrated.
Results:
The electronic nose was also successfully applied to identify unknown samples including sulfur fumed samples and naturally dried samples, high recognition value was obtained. Quantitative analysis of fuming degree of
Pinelliae Rhizoma
was also demonstrated. The method developed is simple and fast, which provides a new quality control method of Chinese herbs from the aspect of odor.
Conclusion:
It has shown that this electronic nose based metal oxide sensor is sensitive to sulfur and sulfides. We suggest that it can serve as a supportive method to detect residual sulfur and sulfides.
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Ultrasound-assisted extraction of total flavonoids from
Aconitum gymnandrum
p. 141
Ling-Li Zheng, Dan Wang, Yuan-Yuan Li, Hong-Yan Peng, Ming-Yong Yuan, Feng Gao
DOI
:10.4103/0973-1296.127364
PMID
:24914295
Background:
Aconitum
gymnandrum
is a Chinese traditional herb used as carminative and analgesic. In this study,
A
.
gymnandrum
was used as an experimental matrix.
Materials and Methods:
Optimized ultrasonic extraction technology of total flavonoids from the
A.
gymnandrum
Maxim was studied by using the methodology of single factor and orthogonal design to study the effects of operation conditions, such as ethanol content, ultrasonic wave power, temperature, ultrasonic wave radiation time, and the ratio of sample weight to solvent volume.
Result:
Through the orthogonal experiment, the optimal extraction conditions were determined as follows: Ultrasonic power 100 W, ultrasonic temperature 45°C, 60% ethyl alcohol, extraction time 30 min, and solid-liquid ratio 1:20.
Conclusion:
Under the optimum parameters, the extraction ratio of total flavonoids from the
A.
gymnandrum
Maxim is about 1.278%.
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Protective effects of
Aegle marmelos
fruit pulp on 2,4,6-trinitrobenzene sulfonic acid-induced experimental colitis
p. 147
Rohit R Ghatule, Manish K Gautam, Shalini Goel, Amit Singh, Vinod K Joshi, Raj K Goel
DOI
:10.4103/0973-1296.127366
PMID
:24914296
Background:
Aegle marmelos
(AM) fruit has been advocated in indigenous system of medicine for the treatment of various gastrointestinal disorders, fever, asthma, inflammations, febrile delirium, acute bronchitis, snakebite, epilepsy, leprosy, myalgia, smallpox, leucoderma, mental illnesses, sores, swelling, thirst, thyroid disorders, tumours and upper respiratory tract infections.
Objective:
The objective of this study was to study the curative effect of 50% ethanol extract of dried fruit pulp of AM (AME) against 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis.
Materials and Methods:
AME (200 mg/kg) was administered orally, once daily for 14 days after TNBS-induced colitis. Rats were given intracolonic normal saline or TNBS alone or TNBS plus oral AME. AME was studied for its
in vitro
antibacterial activity against Gram-negative intestinal bacteria and on TNBS-induced changes in colonic damage, weight and adhesions (macroscopic and microscopic), diarrhea, body weight and colonic levels of free radicals (nitric oxide and lipid peroxidation), antioxidants (superoxide dismutase, catalase and reduced glutathione) and pro-inflammatory marker (myeloperoxidase [MPO]) in rats.
Results:
AME showed antibacterial activity against intestinal pathogens and decreased colonic mucosal damage and inflammation, diarrhea, colonic free radicals and MPO and enhanced body weight and colonic antioxidants level affected by TNBS. The effects of AME on the above parameters were comparable with sulfasalazine, a known colitis protective drug (100 mg/kg, oral).
Conclusion:
AME shows curative effects against TNBS-induced colitis by its antibacterial activity and promoting colonic antioxidants and reducing free radicals and MPO-induced colonic damage.
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Dichlorodiaportinol A - A new chlorine-containing isocoumarin from an endophytic fungus
Trichoderma
sp. 09 from
Myoporum bontioides
A. Gray and its cytotoxic activity
p. 153
Chunyuan Li, Bing Gong, Daniel G Cox, Caili Li, Jinhua Wang, Weijia Ding
DOI
:10.4103/0973-1296.127367
PMID
:24914297
Background:
Myoporum bontioides
A. Gray, an evergreen shrub from the Myoporaceae family, is a commonly used medicinal plant. Many studies have been conducted on the biologically active constituents of whole parts of
M.
bontioides
. However, the endophytes of
M.
bontioides
have not been intensively investigated. A new chlorine-containing isocoumarin, named dichlorodiaportinol A (1) was isolated from the endophytic fungus
Trichoderma
sp. 09 isolated from the root of
M. bontioides
. Its cytotoxic activity against human breast cancer (MCF-7) and human liver cancer (HepG2) cell lines was evaluated.
Materials and Methods:
Different open silica gel column chromatographic techniques with different solvent systems were used for the separation of the constituents of the ethyl acetate extract of the culture broth of the endophytic fungus
Trichoderma
sp. 09. The structure of compound one was identified by analysis of spectroscopic data [one-dimensional (1D), two-dimensional (2D)-nuclear magnetic resonance (NMR), ultraviolet (UV), infrared (IR) and Mass spectrometry (MS)]. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay method was used for the evaluation of cytotoxic activity of compound one against MCF-7 and HepG2 cell lines.
Results:
Compound one was identified as 3-(3,3-dichloro-2,3-dihydroxy-propyl)-8-hydroxy-6- methoxy-isochromen-1-one. It inhibited MCF-7 and HepG2 cell lines, with half maximal inhibitory concentration (IC
50
) values of 17.8 and 39.6 μg/mL, respectively.
Conclusions:
Compound one is a new chlorine-containing isocoumarin with moderate cytotoxic activity against MCF-7 and HepG2 cell lines. Thus, endophytes of
M. bontioides
are worthy of consideration for the development and research of antitumor agents.
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In vitro
anti-telomerase activity of novel lycopene-loaded nanospheres in the human leukemia cell line K562
p. 157
Amir Gharib, Zohreh Faezizadeh
DOI
:10.4103/0973-1296.127368
PMID
:24914298
Background:
Lycopene, a plant carotenoid, has potent effects against the various types of cancer cells. To date, the effect of lycopene in the free and encapsulated forms on the telomerase activity in human leukemia cell line K562 have not been investigated. The aim of the present study was to prepare a novel lycopene-loaded nanosphere and compare its anti-telomearse activity in K562 cell line with those of free lycopene.
Materials
and
Methods:
The lycopene-loaded nanospheres were prepared by nanoprecipitation method. The lycopene entrapment efficacy was measured by high-performance liquid chromatography (HPLC) method. The anti-proliferation effect of the lycopene in the free and encapsulated forms in the different times (0-72 h) and the different doses (0-100 μg/ml) on K562 cell line was studied using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The changes of telomerase activity, following treatment with the lycopene in the free and encapsulated forms, were detected using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay.
Results:
The entrapment efficacy of lycopene was 78.5% ± 2. Treatment of the K562 cell line with lycopene, in particular in encapsulated form, resulted in a significant inhibition of the cell growth and increasing of percentage of apoptotic cells. It has also been observed that the telomerase activity in the lycopene-loaded nanospheres-treated cells was significantly inhibited in a dose and time-dependent manner.
Conclusion:
Our data suggest a novel mechanism in the anti-cancer activity of the lycopene, in particular in encapsulated form, and could be provided a basis for the future development of anti-telomerase therapies.
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Chemical composition of the volatile oil from
Zanthoxylum avicennae
and antimicrobial activities and cytotoxicity
p. 164
Yin Lin, Wei Han, Wei-chen Ge, Ke Yuan
DOI
:10.4103/0973-1296.127369
PMID
:24914299
Background:
Through literature retrieval, there has been no report on the research of the chemical components in
Zanthoxylum avicennae
(Lam.) DC. This paper extracted and determined the chemical components of the volatile oil in
Z. avicennae
, and at the same time, measured and evaluated the bioactivity of the volatile oil in
Z. avicennae
.
Materials and Methods:
We extract the volatile oil in
Z. avicennae
by steam distillation method, determined the chemical composition of the volatile oil by GC-MS coupling technique, and adopt the peak area normalization method to measured the relative percentage of each chemical composition in the volatile oil. Meanwhile, we use the Lethal-to-prawn larva bioactivity experiment to screen the cytotoxicity activities of the volatile oil in
Z. avicennae
, and using the slanting test-tube experiment to determine and evaluate its antibacterial activities
in vitro
for the eight kinds of plant pathogenic fungi in the volatile oil of the
Z. avicennae
.
Results:
The results show that 68 kinds of compounds are determined from the volatile oil of
Z. avicennae
. The determined part takes up 97.89% of the total peak area. The main ingredients in the volatile oil of
Z. avicennae
are sesquiterpenoids and monoterpene. The test results show that the volatile oil in
Z. avicennae
has strong antibacterial activities and cytotoxicity, with the strongest antibacterial activity against the
Rhizoctonia solani
AG1-1A.
Conclusion:
This research results will provide reference data for understanding the chemical composition of the volatile oil in the aromatic plant of
Z. avicennae
and its bioactivity, and for its further development and application.
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Increased acetylcholine esterase activity produced by the administration of an aqueous extract of the seed kernel of
Thevetia peruviana
and its role on acute and subchronic intoxication in mice
p. 171
Rubén Marroquín-Segura, Ricardo Calvillo-Esparza, José Luis Alfredo Mora-Guevara, José Horacio Tovalín-Ahumada, Abigail Aguilar-Contreras, Vicente Jesús Hernández-Abad
DOI
:10.4103/0973-1296.127370
PMID
:24914300
Background:
The real mechanism for
Thevetia
peruviana
poisoning remains unclear. Cholinergic activity is important for cardiac function regulation, however, the effect of
T.
peruviana
on cholinergic activity is not well-known.
Objective:
To study the effect of the acute administration of an aqueous extract of the seed kernel of
T.
peruviana
on the acetylcholine esterase (AChE) activity in CD1 mice as well its implications in the sub-chronic toxicity of the extract.
Materials and Methods:
A dose of 100 mg/kg of the extract was administered to CD1 mice and after 7 days, serum was obtained for ceruloplasmin (CP) quantitation and liver function tests. Another group of mice received a 50 mg/kg dose of the extract 3 times within 1 h time interval and AChE activity was determined for those animals. Heart tissue histological preparation was obtained from a group of mice that received a daily 50 mg/kg dose of the extract by a 30-days period.
Results:
CP levels for the treated group were higher than those for the control group (Student's
t
-test,
P
≤ 0.001). AChE activity in the treated group was significantly higher than the control group (Tukey test, control vs.
T.
peruviana
,
P
≤ 0.001). Heart tissue histological preparations showed leukocyte infiltrates and necrotic areas, consistent with infarcts.
Conclusion:
The increased levels of AChE and the hearth tissue infiltrative lesions induced by the aqueous seed kernel extract of
T.
peruviana
explains in part the poisoning caused by this plant, which can be related to an inflammatory process.
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Optimization of genetic transformation of
Artemisia annua
L. Using
Agrobacterium
for Artemisinin production
p. 176
Elfahmi , Sony Suhandono, Agus Chahyadi
DOI
:10.4103/0973-1296.127372
PMID
:24914301
Background:
Artemisinin, a sesquiterpene lactone endoperoxide isolated from the medicinal plant
Artemisia annua
L., is a choice and effective drug for malaria treatment. Due to the low yield of artemisinin in plants, there is a need to enhance the production of artemisinin from
A. annua
and biotechnological technique may be one of the methods that can be used for the purpose.
Aim:
To study the transformation efficiency of
Agrobacterium tumefaciens
in
A. annua
that could be applied to enhance the production of artemisinin by means of transgenic plants.
Setting and Designs:
The factors influencing
Agrobacterium
-mediated transformation of
A. annua
were explored to optimize the transformation system, which included
A. tumefaciens
strain and effect of organosilicone surfactants. Three strains of
A. tumefaciens
, that is, LBA4404, GV1301, and AGL1 harboring the binary vector pCAMBIA 1303 have been used for transformation. The evaluation was based on transient β-glucuronidase (GUS).
Materials and Methods:
Plant cell cultures were inniatiated from the seeds of
A. annua
using the germination Murashige and Skoog medium.
A. tumefaciens
harboring pCAMBIA were tranformed into the leaves of
A.annua
cultures from 2-week-old-seedling and 2-month-old-seedling for 15 min by vacuum infiltration. Transformation efficiency was determinated by measuring of blue area (GUS expression) on the whole leaves explant using
ImageJ 1.43
software. Two organosilicon surfactants, that is, Silwet L-77 and Silwet S-408 were used to improve the transformation efficiency.
Results:
The transformation frequency with AGL1 strain was higher than GV3101 and LBA4404 which were 70.91, 49.25, and 45.45%, respectively. Effect of organosilicone surfactants, that is, Silwet L-77 and Silwet S-408 were tested on
A. tumefaciens
AGL1 and GV3101 for their level of transient expression, and on
A. rhizogenes
R1000 for its hairy root induction frequency. For AGL1, Silwet S-408 produced higher level of expression than Silwet L-77, were 2.3- and 1.3-fold, respectively. For GV3101, Silwet L-77 was still higher than Silwet S-408, were 1.5- and 1.4-fold, respectively. However, GV3101 produced higher levels of expression than AGL1. The area of GUS expression spots of AGL1, LBA4404, and GV3101 strains was 53.43%, 41.06%, and 30.51%, respectively.
Conclusion:
A. tumefaciens
AGl1 strain was the most effective to be transformed in to
A. annua
than GV3101 and LBA4404 strain. Surfactant Silwet S-408 produced the highest efficiency of transformation.
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Source of variation of isoflavone concentrations in perennial clover species
p. 181
Bronislava Butkute, Butkute Lemežiene, Giedre Dabkeviciene, Valdas Jakštas, Egidijus Vilcinskas, Valdimaras Janulis
DOI
:10.4103/0973-1296.127373
PMID
:24914302
Introduction:
Clover has attracted considerable interest not only as a valuable livestock forage plant, but also as an important source of isoflavones. The current study was aimed to assess the variation of concentration of three isoflavones in clover species grown under a cool temperate climate environment in Lithuania.
Materials and Methods:
Isoflavone contents were quantified in the plant parts of 21 accessions belonging to five perennial species of genus
Trifolium (T. pratense,
T. repens
,
T. medium, T. rubens, and T. pannonicum)
. Daidzein, formononetin, and genistein concentrations were quantified in separate plant parts by reversed-phase high-performance liquid chromatography. The analyte extraction was performed from dried and ground leaves, stems, flowers, and roots. The procedure included acid hydrolysis of isoflavone glycosides to aglycones.
Results:
According to the averaged sum of the three isoflavones quantified in leaves-stems-flowers, the five clover species ranked as follows:
T. medium
(7.54-3.62-2.31 mg/g)
>T. pratense> T. rubens> T. pannonicum> T. repens
(0.191-0.204-0.171 mg/g). The contribution of individual compound to the total isoflavone content depended on the species, accession, and plant part. The major part of the isoflavones is concentrated in leaves or stems; however, there is a great variation also.
Conclusion:
There exists a large variation in the total as well as in individual concentration of isoflavones among the clover species and plant parts and within species. With regard to isoflavone concentration and variability within species, some accessions of
T. medium
and
T. pratense
can be considered a highly promising source of phytoestrogens.
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Multi-component analysis in sun-dried and sulfur-fumigated
Angelicae Sinensis Radix
by single marker quantitation and chemometric discrimination
p. 189
Yajing Lou, Hao Cai, Xiao Liu, Gang Cao, Sicong Tu, Songlin Li, Xiaoqing Ma, Kunming Qin, Baochang Cai
DOI
:10.4103/0973-1296.127374
PMID
:24914303
Background:
A new method has been developed for the simultaneous determination of ferulic acid, senkyunolide A, and Z-ligustilide in
Angelicae Sinensis Radix
before and after sulfur-fumigation using quantitative analysis of multi-components by a single marker (QAMS).
Materials and Methods:
The feasibility and accuracy of QAMS were checked by the external standard method, and various high-performance liquid chromatographic instruments and chromatographic conditions were investigated to verify its applicability. Using ferulic acid as the internal reference substance, and the contents of senkyunolide A and Z-ligustilide were calculated according to relative correction factors by high-performance liquid chromatography. Meanwhile, the influence of sulfur-fumigation on these chemical components in
Angelicae Sinensis Radix
were evaluated and discriminated by chromatographic fingerprint and chemometrics.
Results:
There was no significant difference observed between the QAMS method and the external standard method. Furthermore, sulfur-fumigation reduced the contents of ferulic acid, senkyunolide A, and Z-ligustilide in
Angelicae Sinensis Radix
by some degree, and the sun-drying and sulfur-fumigation processing could be easily discriminated by chromatographic fingerprint and chemometrics.
Conclusion:
QAMS is a convenient and accurate approach to analyzing multi-component when reference substances are unavailable, simultaneously, chemometrics is an effective way to discriminate sun-dried and sulfur-fumigated
Angelicae Sinensis Radix
.
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Rapid, sensitive, and validated UPLC/Q-TOF-MS method for quantitative determination of vasicine in
Adhatoda vasica
and its
in vitro
culture
p. 198
Garg Madhukar, Ennus Tajuddin Tamboli, Parveen Rabea, SH Ansari, MZ Abdin, Ahmad Sayeed
DOI
:10.4103/0973-1296.127375
PMID
:24914304
Background:
Adhatoda vasica
a perennial herb has been used in Ayurvedic and Unani system of medicines since last 2000 years and has been employed for the treatment of respiratory tract ailments.
Objective:
To develop and validate new, rapid, and highly sensitive high throughput ultra-performance liquid chromatography/quadrupole-time-of-flight mass-spectrometry (UPLC/Q-TOF-MS) method for the quantitative estimation of vasicine in the leaves and to establish
in vitro
cultures of
Adhatoda vasica
for production of vasicine.
Materials and Methods:
The chromatographic separation was achieved on a Waters ACQUITY UPLC
TM
BEH C8 (100.0 × 2.1 mm; 1.7 μm) column packing using isocratic mobile phase consisting of acetonitrile: 20 mM ammonium acetate (90:10; v/v) in a multiple reactions monitoring mode using the transitions m/z 189.09 → 171.08 for vasicine.
Results:
The vasicine was eluted at 2.58 ± 0.05 min and established a dynamic range of linearity over the concentration range of 1-1000 ng/ml (
r
2
= 0.999 ± 0.0005). The lower limit of detection and quantification was 0.68 and 1.0 ng/ml, respectively. There was no significant difference observed in the content of vasicine (0.92-1.04%w/w) among the eleven samples collected from different locations of India. The
in vitro
cultures developed showed that addition of extra 28 mM KNO
3
and 100 mM NaCl in MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) + benzyladenine (BA) + indole acetic acid (IAA) (1 ppm each) produces faster biomass and higher amount of quinazoline alkaloids.
Conclusion:
Rapid, efficient, and sensitive UPLC/Q-TOF-MS method was developed for the estimation of vasicine and an efficient protocol for development of
in vitro
cultures was proposed, which can be used at large scale for industrial production of vasicine using bioreactors.
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