Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 17  |  Issue : 74  |  Page : 307--314

Carthami flos induces apoptosis by activating caspases and regulating mitogen-activated protein kinase and reactive oxygen species signaling pathways in AGS human gastric cancer cells


Song Ee Han1, Min Ji Kwon1, Jeong Nam Kim1, Eun Yeong Lim2, Yun Tai Kim2, Byung Joo Kim1 
1 Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea
2 Division of Food Functionality, Korea Food Research Institute, Wanju; Department of Food Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea

Correspondence Address:
Byung Joo Kim
Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Beomeori, Mulgeum-eup, Yangsan, Gyeongsangnam-do, 50612
Republic of Korea

Background: Carthami flos (CF) is a traditional medicine used to treat various diseases, especially cancer therapy. Objectives: The mechanisms of cell death induction by CF were investigated in AGS human gastric adenocarcinoma cells. Materials and Methods: Cell viability assay, cell cycle analysis, caspase activity assay, western blotting, and reactive oxygen species (ROS) assay were used to check the anti-cancer effects of CF on AGS cells. Results: Treatment with CF (100500 μg/mL) inhibited AGS cell proliferation and increased the ratio of the subG1 phase of the cell cycle. CF induced cell death was associated with a reduction in Bcl-2 and an increase in Bax levels. Moreover, expression of the apoptosismediating surface antigen (FAS) was increased. CF activated caspase-3 and -9. In addition, it regulated the activation of mitogen-activated protein kinases and increased intracellular ROS generation. Conclusion: These findings suggest that CF induced apoptosis in AGS cells and could thus, serve as a novel anticancer agent to promote apoptosis of gastric cancer cells.


How to cite this article:
Han SE, Kwon MJ, Kim JN, Lim EY, Kim YT, Kim BJ. Carthami flos induces apoptosis by activating caspases and regulating mitogen-activated protein kinase and reactive oxygen species signaling pathways in AGS human gastric cancer cells.Phcog Mag 2021;17:307-314


How to cite this URL:
Han SE, Kwon MJ, Kim JN, Lim EY, Kim YT, Kim BJ. Carthami flos induces apoptosis by activating caspases and regulating mitogen-activated protein kinase and reactive oxygen species signaling pathways in AGS human gastric cancer cells. Phcog Mag [serial online] 2021 [cited 2022 Dec 8 ];17:307-314
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2021;volume=17;issue=74;spage=307;epage=314;aulast=Han;type=0