Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 17  |  Issue : 74  |  Page : 231--235

Costunolide attenuates oxygen-glucose deprivation/reoxygenation-induced apoptosis in mouse brain slice through inhibiting caspase expression


Huixia Ma1, Yafei Zhu2, Zhengjun Zhang3, Xinhui Zhang1, Qipeng Zhao1 
1 Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education; Department of Pharmacology, Ningxia Medical University, Yinchuan, China
2 Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education; College of Basic Medicine, Ningxia Medical University, Yinchuan, China
3 Department of Cardiology, General Hospital of Ningxia Medical University, Yinchuan, China

Correspondence Address:
Qipeng Zhao
Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004; Department of Pharmacology, Ningxia Medical University, Yinchuan 750004
China
Xinhui Zhang
Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004; Department of Pharmacology, Ningxia Medical University, Yinchuan 750004
China

Background: Costunolide (Co) has anti-tumor, anti-inflammation, and anti-ulcer effects, it has the budding effect of anti-apoptosis. This study was intended to explicate its inhibitory effect on caspase in a mice brain slices injury model. Materials and Methods: The maestro 11.1 software was employed to envisage the binding sites of Co with Caspase-3, Caspase-9, and Caspase-7. Oxygen-glucose deprivation/reoxygenation (OGD/R) method was employed to persuade mouse brain slice injury in vitro. Purpose of lactate dehydrogenase (LDH) in culture medium and 2,3,5-triphenyl-tetrazolium chloride (TTC) staining of brain slices for the assessment of injury degree. The expression of Cytochrome c, Caspase-9, Caspase-7, Caspase-3, Bcl-2, and Bax was studied by Western blot method. Results: The results of docking displayed that Co had binding sites withe Caspase-9, Caspase-7, and Caspase-3. Compared with OGD/R, Co could diminution the LDH levels, upsurge the TTC staining intensity, augment Bcl-2 expression level and inhibit Caspase-3, Caspase-9, Caspase-7, Bax, and Cytochrome c expression levels. Conclusion: These results recommended that Co has latent neuroprotective activities by inhibiting caspase expression.


How to cite this article:
Ma H, Zhu Y, Zhang Z, Zhang X, Zhao Q. Costunolide attenuates oxygen-glucose deprivation/reoxygenation-induced apoptosis in mouse brain slice through inhibiting caspase expression.Phcog Mag 2021;17:231-235


How to cite this URL:
Ma H, Zhu Y, Zhang Z, Zhang X, Zhao Q. Costunolide attenuates oxygen-glucose deprivation/reoxygenation-induced apoptosis in mouse brain slice through inhibiting caspase expression. Phcog Mag [serial online] 2021 [cited 2021 Sep 25 ];17:231-235
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2021;volume=17;issue=74;spage=231;epage=235;aulast=Ma;type=0